Low-temperature-related growth and photosynthetic performance of alloplasmic tomato (Lycopersicon esculentum Mill.) with chloroplasts from L-hirsutum Humb. & Bonpl.

O Dolstra*, JH Venema, PJ Groot, PR van Hasselt

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    5 Citations (Scopus)

    Abstract

    Growth and photosynthetic performance were analyzed in alloplasmic tomato at a high- (25/17degreesC; HTR) and low-temperature regime (12/6degreesC; LTR) in order to establish the role of cytoplasmic variation on low-temperature tolerance of tomato ( Lycopersicon esculentum Mill.). Four alloplasmic tomato lines, containing the nuclear genome of tomato and the plastome of L. hirsutum LA 1777 Humb. & Bonpl., an accession collected at high-altitude in Peru, were reciprocally crossed with 11 tomato entries with a high inbreeding level and a wide genetic variation, resulting in a set of 44 reciprocal crosses. Irrespective of growth temperature, alloplasmic families with alien chloroplasts of L. hirsutum (h) were on average characterized by a high shoot biomass, a large leaf area, and a low specific leaf area in comparison with their euplasmic counterparts. These results do not directly point to an advantageous effect of h-chloroplasts on biomass accumulation at low temperature but rather towards a small general beneficial effect on growth and/or distribution of assimilates. Significant chloroplast-related differences in photosynthetic performance, however, were not detected at both temperature regimes, indicating that h-chloroplasts can properly function in a variable nuclear background of L. esculentum. It is concluded that chloroplast substitution is not an effective method for breeding tomato plants with improved low-temperature tolerance.

    Original languageEnglish
    Pages (from-to)407-421
    Number of pages15
    JournalEuphytica
    Volume124
    Issue number3
    Publication statusPublished - 2002

    Keywords

    • alien cytoplasmic variation
    • chloroplasts
    • low-temperature tolerance
    • Lycopersicon esculentum
    • L. hirsutum
    • tomato
    • CHLOROPHYLL FLUORESCENCE
    • NUCLEOCYTOPLASMIC INCOMPATIBILITY
    • LOW-LIGHT
    • TOLERANCE
    • GENOME
    • PLASTOME
    • PLANTS
    • CYBRID
    • COLD
    • PERUVIANUM

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