Lymphatic uptake and biodistribution of liposomes after subcutaneous injection - IV. Fate of liposomes in regional lymph nodes

C Oussoren*, G Scherphof, JJ van der Want, N van Rooijen, G Storm

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

67 Citations (Scopus)

Abstract

The ability of clodronate-containing liposomes to deplete lymph nodes of macrophages was used as a tool to investigate the fate of liposomes in regional lymph nodes after subcutaneous (s.c.) administration. Reduced lymph node localization of liposomes in macrophage-depleted lymph nodes confirmed that phagocytosis by macrophages plays an important role in lymph node retention of liposomes. Depletion of macrophages had less effect on lymph node localization of small liposomes than on the lymph node localization of large liposomes. Inclusion of distearoylphosphatidylethanolamine (DSPE)-poly(ethyleneglycol) (PEG-PE) into the liposomes, which is known to oppose macrophage uptake, did not affect lymph node localization in macrophage-depleted or control lymph nodes, We conclude that PEG-Liposomes retained by lymph nodes are also taken up by lymph node macrophages. Morphological observations visualizing the uptake of PEG-liposomes by lymph node macrophages support this conclusion. (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)259-272
Number of pages14
JournalBiochimica et Biophysica Acta-Biomembranes
Volume1370
Issue number2
Publication statusPublished - 13-Mar-1998

Keywords

  • liposome
  • subcutaneous
  • macrophage
  • depletion
  • clodronate
  • colloidal gold
  • MONONUCLEAR PHAGOCYTE SYSTEM
  • LIVER ENDOTHELIAL-CELLS
  • MEDIATED DEPLETION
  • RAT
  • MACROPHAGES
  • ELIMINATION
  • CLEARANCE
  • ETHER

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