Macrophage phagocytic activity toward adhering staphylococci on cationic and patterned hydrogel coatings versus common biomaterials

Joana Da Silva Domingues, Steven Roest, Yi Wang, Henny C. van der Mei*, Matthew Libera, Theo G. van Kooten, Henk J. Busscher

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

27 Citations (Scopus)

Abstract

Biomaterial-associated-infection causes failure of biomaterial implants. Many new biomaterials have been evaluated for their ability to inhibit bacterial colonization and stimulate tissue-cell-integration, but neglect the role of immune cells. This paper compares macrophage phagocytosis of adhering Staphylococcus aureus on cationic-coatings and patterned poly(ethylene)glycol-hydrogels versus common biomaterials and stainless steel in order to identify surface conditions that promote clearance of adhering bacteria. Staphylococci were allowed to adhere and grow on the materials in a parallel-plate-flow-chamber, after which murine macrophages were introduced. From the decrease in the number of adhering staphylococci, phagocytosis-rates were calculated, and total macrophage displacements during an experiment determined. Hydrophilic surfaces had the lowest phagocytosis-rates, while common biomaterials had intermediate phagocytosis-rates. Patterning of poly(ethylene)glycol-hydrogel coatings increased phagocytosis-rates to the level of common biomaterials, while on cationic-coatings phagocytosis-rates remained relatively low. Likely, phagocytosis-rates on cationic coatings are hampered relative to common biomaterials through strong electrostatic binding of negatively-charged macrophages and staphylococci. On polymeric biomaterials and glass, phagocytosis-rates increased with macrophage displacement, while both parameters increased with biomaterial surface hydrophobicity. Thus hydrophobicity is a necessary surface condition for effective phagocytosis. Concluding, next-generation biomaterials should account for surface effects on phagocytosis in order to enhance the ability of these materials to resist biomaterial-associated-infection. (C) 2015 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalActa Biomaterialia
Volume18
DOIs
Publication statusPublished - May-2015

Keywords

  • Biomaterial-associated infection
  • Poly(ethylene)glycol coatings
  • Cationic coatings
  • Water contact angles
  • Phagocytosis
  • GRAM-POSITIVE BACTERIA
  • LIPOTEICHOIC ACID
  • TISSUE INTEGRATION
  • MICROBIAL ADHESION
  • BIOFILM FORMATION
  • J774 MACROPHAGES
  • STAINLESS-STEEL
  • CELL-ADHESION
  • NITRIC-OXIDE
  • SURFACES

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