Maintaining Sidedness and Fluidity in Cell Membrane Coatings Supported on Nano-Particulate and Planar Surfaces

Sidi Liu, Yuanfeng Li, Linqi Shi, Jian Liu, Yijin Ren, Jon D. Laman, Henny C. van der Mei*, Henk J. Busscher*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Supported cell membrane coatings meet many requirements set to bioactive nanocarriers and materials, provided sidedness and fluidity of the natural membrane are maintained upon coating. However, the properties of a support-surface responsible for maintaining correct sidedness and fluidity are unknown. Here, we briefly review the properties of natural membranes and membrane-isolation methods, with focus on the asymmetric distribution of functional groups in natural membranes (sidedness) and the ability of molecules to float across a membrane to form functional domains (fluidity). This review concludes that hydrophilic sugar-residues of glycoproteins in the outer-leaflet of cell membranes direct the more hydrophobic inner-leaflet towards a support-surface to create a correctly-sided membrane coating, regardless of electrostatic double-layer interactions. On positively-charged support-surfaces however, strong, electrostatic double-layer attraction of negatively-charged membranes can impede homogeneous coating. In correctly-sided membrane coatings, fluidity is maintained regardless of whether the surface carries a positive or negative charge. However, membranes are frozen on positively-charged, highly-curved, small nanoparticles and localized nanoscopic structures on a support-surface. This leaves an unsupported membrane coating in between nanostructures on planar support-surfaces that is in dual-sided contact with its aqueous environment, yielding enhanced fluidity in membrane coatings on nanostructured, planar support-surfaces as compared with smooth ones.
Original languageEnglish
Pages (from-to)344-355
Number of pages12
JournalBioactive Materials
Volume32
DOIs
Publication statusPublished - Feb-2024

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