MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OF ISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS

GW SANDKER; B WEERT; W KUIPERS; MJH SLOOFF; DKF MEIJER; GMM GROOTHUIS; M.T. Merema

MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OF ISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS

GW SANDKER, B WEERT, W KUIPERS, MJH SLOOFF, DKF MEIJER, GMM GROOTHUIS, M.T. Merema

Biopharmaceuticals, Discovery, Design and Delivery (BDDD)

Research output: Contribution to journal › Comment/Letter to the editor › Academic › peer-review

16 Citations (Scopus)

Abstract

Rat hepatocytes were preserved for 24hr with high recovery and good maintenance of viability and transport function both in University of Wisconsin (UW) solution and in various simplified UW solutions. Cell quality is somewhat affected after 48 hr of preservation in both the original UW solution and the simplified solutions. ATP content and uptake rate of taurocholic acid are more sensitive markers of cell viability than Trypan blue exclusion or the MTT test. A much less expensive solution than UW, containing only K+-lactobionate, KH2PO4, MgSO4 and raffinose, can be used successfully for preservation of rat hepatocytes for 24 hr for drug transport studies.

Original language	English
Pages (from-to)	2093-2096
Number of pages	4
Journal	Biochemical Pharmacology
Volume	46
Issue number	11
Publication status	Published - 3-Dec-1993

Keywords

SIMPLE COLD-STORAGE
UW-SOLUTION
LIVER PRESERVATION
KIDNEY-PRESERVATION
HYDROXYETHYL STARCH
HYPOTHERMIC PRESERVATION
METABOLIC FUNCTION
CELL VIABILITY
CANINE LIVER
EURO-COLLINS

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title = "MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OF ISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS",

abstract = "Rat hepatocytes were preserved for 24hr with high recovery and good maintenance of viability and transport function both in University of Wisconsin (UW) solution and in various simplified UW solutions. Cell quality is somewhat affected after 48 hr of preservation in both the original UW solution and the simplified solutions. ATP content and uptake rate of taurocholic acid are more sensitive markers of cell viability than Trypan blue exclusion or the MTT test. A much less expensive solution than UW, containing only K+-lactobionate, KH2PO4, MgSO4 and raffinose, can be used successfully for preservation of rat hepatocytes for 24 hr for drug transport studies.",

keywords = "SIMPLE COLD-STORAGE, UW-SOLUTION, LIVER PRESERVATION, KIDNEY-PRESERVATION, HYDROXYETHYL STARCH, HYPOTHERMIC PRESERVATION, METABOLIC FUNCTION, CELL VIABILITY, CANINE LIVER, EURO-COLLINS",

author = "GW SANDKER and B WEERT and W KUIPERS and MJH SLOOFF and DKF MEIJER and GMM GROOTHUIS and M.T. Merema",

year = "1993",

month = dec,

day = "3",

language = "English",

volume = "46",

pages = "2093--2096",

journal = "Biochemical Pharmacology",

issn = "0006-2952",

publisher = "PERGAMON-ELSEVIER SCIENCE LTD",

number = "11",

}

TY - JOUR

T1 - MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OF ISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS

AU - SANDKER, GW

AU - WEERT, B

AU - KUIPERS, W

AU - SLOOFF, MJH

AU - MEIJER, DKF

AU - GROOTHUIS, GMM

AU - Merema, M.T.

PY - 1993/12/3

Y1 - 1993/12/3

N2 - Rat hepatocytes were preserved for 24hr with high recovery and good maintenance of viability and transport function both in University of Wisconsin (UW) solution and in various simplified UW solutions. Cell quality is somewhat affected after 48 hr of preservation in both the original UW solution and the simplified solutions. ATP content and uptake rate of taurocholic acid are more sensitive markers of cell viability than Trypan blue exclusion or the MTT test. A much less expensive solution than UW, containing only K+-lactobionate, KH2PO4, MgSO4 and raffinose, can be used successfully for preservation of rat hepatocytes for 24 hr for drug transport studies.

AB - Rat hepatocytes were preserved for 24hr with high recovery and good maintenance of viability and transport function both in University of Wisconsin (UW) solution and in various simplified UW solutions. Cell quality is somewhat affected after 48 hr of preservation in both the original UW solution and the simplified solutions. ATP content and uptake rate of taurocholic acid are more sensitive markers of cell viability than Trypan blue exclusion or the MTT test. A much less expensive solution than UW, containing only K+-lactobionate, KH2PO4, MgSO4 and raffinose, can be used successfully for preservation of rat hepatocytes for 24 hr for drug transport studies.

KW - SIMPLE COLD-STORAGE

KW - UW-SOLUTION

KW - LIVER PRESERVATION

KW - KIDNEY-PRESERVATION

KW - HYDROXYETHYL STARCH

KW - HYPOTHERMIC PRESERVATION

KW - METABOLIC FUNCTION

KW - CELL VIABILITY

KW - CANINE LIVER

KW - EURO-COLLINS

M3 - Comment/Letter to the editor

SN - 0006-2952

VL - 46

SP - 2093

EP - 2096

JO - Biochemical Pharmacology

JF - Biochemical Pharmacology

IS - 11

ER -

MAINTENANCE OF VIABILITY AND TRANSPORT FUNCTION AFTER PRESERVATION OF ISOLATED RAT HEPATOCYTES IN VARIOUS SIMPLIFIED UNIVERSITY-OF-WISCONSIN SOLUTIONS

Abstract

Keywords

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