@article{1f18f0be0ebe49f897d714df72a9bb8a,
title = "Mapping diversity in African trypanosomes using high resolution spatial proteomics",
abstract = "African trypanosomes are dixenous eukaryotic parasites that impose a significant human and veterinary disease burden on sub-Saharan Africa. Diversity between species and life-cycle stages is concomitant with distinct host and tissue tropisms within this group. Here, the spatial proteomes of two African trypanosome species, Trypanosoma brucei and Trypanosoma congolense, are mapped across two life-stages. The four resulting datasets provide evidence of expression of approximately 5500 proteins per cell-type. Over 2500 proteins per cell-type are classified to specific subcellular compartments, providing four comprehensive spatial proteomes. Comparative analysis reveals key routes of parasitic adaptation to different biological niches and provides insight into the molecular basis for diversity within and between these pathogen species.",
author = "Moloney, {Nicola M.} and Konstantin Barylyuk and Eelco Tromer and Crook, {Oliver M.} and Breckels, {Lisa M.} and Lilley, {Kathryn S.} and Waller, {Ross F.} and Paula MacGregor",
note = "Funding Information: This work was funded by a BBSRC David Phillips Fellowship (BB/P010849/1) to P.M. and an Isaac Newton Trust, Wellcome Trust, University of Cambridge Joint Research Grant to P.M.; K.B. was supported by a Wellcome Trust Investigator award (214298/Z/18/Z) to R.F.W. and a Leverhulme Early Career Fellowship (ECF-2015-562) to K.B., which was jointly funded by the Leverhulme Trust and Isaac Newton Trust. E.T. acknowledges support from the Dutch Science Organisation (VI.Veni.202.223). We thank: Mark Carrington (University of Cambridge) for provision of antibodies and cells, James Bangs (University at Buffalo) for provision of antibodies, Catarina Gadelha (University of Nottingham) for provision of cells, Mike Deery (Cambridge Centre for Proteomics) for performing mass spectrometry analysis, and Yagnesh Umrania (Cambridge Centre for Proteomics) for assistance with proteomics analysis. Funding Information: This work was funded by a BBSRC David Phillips Fellowship (BB/P010849/1) to P.M. and an Isaac Newton Trust, Wellcome Trust, University of Cambridge Joint Research Grant to P.M.; K.B. was supported by a Wellcome Trust Investigator award (214298/Z/18/Z) to R.F.W. and a Leverhulme Early Career Fellowship (ECF-2015-562) to K.B., which was jointly funded by the Leverhulme Trust and Isaac Newton Trust. E.T. acknowledges support from the Dutch Science Organisation (VI.Veni.202.223). We thank: Mark Carrington (University of Cambridge) for provision of antibodies and cells, James Bangs (University at Buffalo) for provision of antibodies, Catarina Gadelha (University of Nottingham) for provision of cells, Mike Deery (Cambridge Centre for Proteomics) for performing mass spectrometry analysis, and Yagnesh Umrania (Cambridge Centre for Proteomics) for assistance with proteomics analysis. Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
month = dec,
doi = "10.1038/s41467-023-40125-z",
language = "English",
volume = "14",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}