Abstract
The invention relates to the field of enzyme engineering and biocatalysis, in particular to alcohol oxidases and their application in the selective oxidation of diols for the production of industrially useful oxidation products thereof, such as precursors for biodegradable polyesters. Provided is a method for the selective oxidation of a diol substrate, comprising subjecting a diol substrate of the formula HO-CH2-CH2-X-CH2-CH2-OH, wherein X = O, S or CH2, to a FAD-containing alcohol oxidase (AOX) enzyme of the class EC 1.1.3.13 or EC 1.1.3.17. or a mutant thereof, under conditions allowing lor the double oxidation of one hydroxyl moiety of the diol substrate into an oxidation product.
| Original language | English |
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| Patent number | WO2021071356 |
| Priority date | 08/10/2019 |
| Filing date | 07/10/2020 |
| Publication status | Published - 15-Apr-2021 |