Measurement of insulin-like growth factor-1 and insulin-like growth factor binding protein-3 after delayed separation of whole blood samples

Hermien Hartog, Winette T. A. van der Graaf, Jelle Wesseling, Eveline van der Veer, H. Marike Boezen*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)


Objectives: Epidemiological studies benefit from unbiased blood specimens collected with minimal cost and effort of blood collection and storage. We evaluated the stability of IGF-1 and IGFBP-3 in whole blood samples stored at room temperature to justify delays in blood processing.

Design and methods: Total IGF-1 and IGFBP-3 levels were measured in EDTA plasma (n = 12), heparin plasma (n = 12) and serum (n = 10) samples of healthy volunteers after blood processing delays up till 14 days. Stability of measured IGF-1 and IGFBP-3 levels was tested by paired t-test and a linear mixed effect model.

Results: Longitudinal analysis showed that IGF-1 levels were not significantly affected by blood processing delays in EDTA tubes (P = 0.18) and IGFBP-3 levels were marginally stable (p = 0.06). In heparin plasma and serum, however, IGF-1 increased over time of delayed processing and IGFBP-3 levels tended to decrease (p

Conclusion: Total IGF-1 and IGFBP-3 levels are stable in whole blood collected in EDTA tubes at room temperature up till 7 days, allowing a delay in blood processing to reduce costs in large multi-center studies. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)636-639
Number of pages4
JournalClinical biochemistry
Issue number7-8
Publication statusPublished - May-2008


  • IGF-1
  • IGFBP-3
  • storage
  • stability
  • serum
  • plasma
  • whole blood
  • TIME
  • RISK

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