Measurement of slowly exchanging45Ca in rat aorta without using EGTA or lanthanum and its application to quantify the effects of the calcium entry blockers nifedipine and verapamil

D. Wermelskirchen; D. Wilhelm; B. Wilffert; B.L. Pegram; J. Hunter; U. Nebel; Thies Peters

Measurement of slowly exchanging45Ca in rat aorta without using EGTA or lanthanum and its application to quantify the effects of the calcium entry blockers nifedipine and verapamil

D. Wermelskirchen, D. Wilhelm, B. Wilffert, B.L. Pegram, J. Hunter, U. Nebel, Thies Peters

Reproductive Origins of Adult Health and Disease (ROAHD)

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Abstract

In rat aortic strips a method was developed to measure a fraction of slowly exchanging45Ca, which correlates with contraction and the cytosolic Ca pool that is enhanced by K+-induced depolarization. In this method no EGTA or lanthanum are used, but the strips are washed for 45 min with a Tyrode solution at 4°C. The K+depolarization induced increase in45Ca and contraction was concentration-dependently inhibited by verapamil and nifedipine. Since lanthanum and EGTA affect cellular membranes, this method may allow a more physiological approach to the measurements of slowly exchanging45Ca.

Original language	English
Pages (from-to)	84-90
Number of pages	7
Journal	Pharmacology
Volume	36
Issue number	2
Publication status	Published - 14-Nov-1988

Keywords

calcium
egtazic acid
lanthanum
nifedipine
verapamil
animal cell
aorta
depolarization
ion exchange
male
methodology
nonhuman
rat
smooth muscle contraction
procedures

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title = "Measurement of slowly exchanging45Ca in rat aorta without using EGTA or lanthanum and its application to quantify the effects of the calcium entry blockers nifedipine and verapamil",

abstract = "In rat aortic strips a method was developed to measure a fraction of slowly exchanging45Ca, which correlates with contraction and the cytosolic Ca pool that is enhanced by K+-induced depolarization. In this method no EGTA or lanthanum are used, but the strips are washed for 45 min with a Tyrode solution at 4°C. The K+depolarization induced increase in45Ca and contraction was concentration-dependently inhibited by verapamil and nifedipine. Since lanthanum and EGTA affect cellular membranes, this method may allow a more physiological approach to the measurements of slowly exchanging45Ca.",

keywords = "calcium, egtazic acid, lanthanum, nifedipine, verapamil, animal cell, aorta, depolarization, ion exchange, male, methodology, nonhuman, rat, smooth muscle contraction, procedures",

author = "D. Wermelskirchen and D. Wilhelm and B. Wilffert and B.L. Pegram and J. Hunter and U. Nebel and Thies Peters",

year = "1988",

month = nov,

day = "14",

language = "English",

volume = "36",

pages = "84--90",

journal = "Pharmacology",

issn = "0031-7012",

number = "2",

}

Measurement of slowly exchanging45Ca in rat aorta without using EGTA or lanthanum and its application to quantify the effects of the calcium entry blockers nifedipine and verapamil. / Wermelskirchen, D.; Wilhelm, D.; Wilffert, B. et al.
In: Pharmacology, Vol. 36, No. 2, 14.11.1988, p. 84-90.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Measurement of slowly exchanging45Ca in rat aorta without using EGTA or lanthanum and its application to quantify the effects of the calcium entry blockers nifedipine and verapamil

AU - Wermelskirchen, D.

AU - Wilhelm, D.

AU - Wilffert, B.

AU - Pegram, B.L.

AU - Hunter, J.

AU - Nebel, U.

AU - Peters, Thies

PY - 1988/11/14

Y1 - 1988/11/14

N2 - In rat aortic strips a method was developed to measure a fraction of slowly exchanging45Ca, which correlates with contraction and the cytosolic Ca pool that is enhanced by K+-induced depolarization. In this method no EGTA or lanthanum are used, but the strips are washed for 45 min with a Tyrode solution at 4°C. The K+depolarization induced increase in45Ca and contraction was concentration-dependently inhibited by verapamil and nifedipine. Since lanthanum and EGTA affect cellular membranes, this method may allow a more physiological approach to the measurements of slowly exchanging45Ca.

AB - In rat aortic strips a method was developed to measure a fraction of slowly exchanging45Ca, which correlates with contraction and the cytosolic Ca pool that is enhanced by K+-induced depolarization. In this method no EGTA or lanthanum are used, but the strips are washed for 45 min with a Tyrode solution at 4°C. The K+depolarization induced increase in45Ca and contraction was concentration-dependently inhibited by verapamil and nifedipine. Since lanthanum and EGTA affect cellular membranes, this method may allow a more physiological approach to the measurements of slowly exchanging45Ca.

KW - calcium

KW - egtazic acid

KW - lanthanum

KW - nifedipine

KW - verapamil

KW - animal cell

KW - aorta

KW - depolarization

KW - ion exchange

KW - male

KW - methodology

KW - nonhuman

KW - rat

KW - smooth muscle contraction

KW - procedures

M3 - Article

SN - 0031-7012

VL - 36

SP - 84

EP - 90

JO - Pharmacology

JF - Pharmacology

IS - 2

ER -

Measurement of slowly exchanging45Ca in rat aorta without using EGTA or lanthanum and its application to quantify the effects of the calcium entry blockers nifedipine and verapamil

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