Mechanical ventilation with high tidal volumes attenuates myocardial dysfunction by decreasing cardiac edema in a rat model of LPS-induced peritonitis

Lonneke Smeding*, Frans B. Plotz, Regis R. Lamberts, Willem J. van der Laarse, Martin C. J. Kneyber, A. B. Johan Groeneveld

*Corresponding author for this work

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Abstract

Background: Injurious mechanical ventilation (MV) may augment organ injury remote from the lungs. During sepsis, myocardial dysfunction is common and increased endothelial activation and permeability can cause myocardial edema, which may, among other factors, hamper myocardial function. We investigated the effects of MV with injuriously high tidal volumes on the myocardium in an animal model of sepsis.

Methods: Normal rats and intraperitoneal (i.p.) lipopolysaccharide (LPS)-treated rats were ventilated with low (6 ml/kg) and high (19 ml/kg) tidal volumes (Vt) under general anesthesia. Non-ventilated animals served as controls. Mean arterial pressure (MAP), central venous pressure (CVP), cardiac output (CO) and pulmonary plateau pressure (P-plat) were measured. Ex vivo myocardial function was measured in isolated Langendorff-perfused hearts. Cardiac expression of endothelial vascular cell adhesion molecule (VCAM)-1 and edema were measured to evaluate endothelial inflammation and leakage.

Results: MAP decreased after LPS-treatment and Vt-dependently, both independent of each other and with interaction. MV Vt-dependently increased CVP and Pplat and decreased CO. LPS-induced peritonitis decreased myocardial function ex vivo but MV attenuated systolic dysfunction Vt-dependently. Cardiac endothelial VCAM-1 expression was increased by LPS treatment independent of MV. Cardiac edema was lowered Vt-dependently by MV, particularly after LPS, and correlated inversely with systolic myocardial function parameters ex vivo.

Conclusion: MV attenuated LPS-induced systolic myocardial dysfunction in a Vt-dependent manner. This was associated with a reduction in cardiac edema following a lower transmural coronary venous outflow pressure during LPS-induced coronary inflammation.

Original languageEnglish
Article number23
Number of pages8
JournalRespiratory Research
Volume13
DOIs
Publication statusPublished - 20-Mar-2012

Keywords

  • Ventilator-induced lung injury
  • Endothelial permeability
  • Myocardial depression
  • Myocardial edema
  • INDUCED LUNG INJURY
  • END-EXPIRATORY PRESSURE
  • MULTIPLE ORGAN DYSFUNCTION
  • LEFT-VENTRICULAR FUNCTION
  • INFLAMMATORY RESPONSES
  • SEPSIS
  • PERMEABILITY
  • ENDOTHELIUM
  • EXPRESSION
  • MEDIATORS

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