Mesenchymal Stromal Cells as Anti-Inflammatory and Regenerative Mediators for Donor Kidneys During Normothermic Machine Perfusion

Jesus Maria Sierra-Parraga*, Marco Eijken, James Hunter, Cyril Moers, Henri Leuvenink, Bjarne Moller, Rutger J. Ploeg, Carla C. Baan, Bente Jespersen, Martin J. Hoogduijn

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

22 Citations (Scopus)

Abstract

There is great demand for transplant kidneys for the treatment of end-stage kidney disease patients. To expand the donor pool, organs from older and comorbid brain death donors, so-called expanded criteria donors (ECD), as well as donation after circulatory death donors, are considered for transplantation. However, the quality of these organs may be inferior to standard donor organs. A major issue affecting graft function and survival is ischemia/reperfusion injury, which particularly affects kidneys from deceased donors. The development of hypothermic machine perfusion has been introduced in kidney transplantation as a preservation technique and has improved outcomes in ECD and marginal organs compared to static cold storage. Normothermic machine perfusion (NMP) is the most recent evolution of perfusion technology and allows assessment of the donor organ before transplantation. The possibility to control the content of the perfusion fluid offers opportunities for damage control and reparative therapies during machine perfusion. Mesenchymal stromal cells (MSC) have been demonstrated to possess potent regenerative properties via the release of paracrine effectors. The combination of NMP and MSC administration at the same time is a promising procedure in the field of transplantation. Therefore, the MePEP consortium has been created to study this novel modality of treatment in preparation for human trials. MePEP aims to assess the therapeutic effects of MSC administered ex vivo by NMP in the mechanisms of injury and repair in a porcine kidney autotransplantation model.

Original languageEnglish
Pages (from-to)1162-1170
Number of pages9
JournalStem cells and development
Volume26
Issue number16
DOIs
Publication statusPublished - 15-Aug-2017

Keywords

  • MSC
  • endothelial cells
  • machine perfusion
  • regeneration
  • organ transplantation
  • ISCHEMIA-REPERFUSION INJURY
  • EXPANDED CRITERIA DONORS
  • ENDOTHELIAL PROGENITOR CELLS
  • RANDOMIZED CONTROLLED-TRIAL
  • HEPATOCYTE GROWTH-FACTOR
  • DELAYED GRAFT FUNCTION
  • REGULATORY T-CELLS
  • STEM-CELLS
  • BONE-MARROW
  • RENAL-TRANSPLANTATION

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