Metabolic engineering and synthetic biology employing Lactococcus lactis and Bacillus subtilis cell factories

Amanda Y. van Tilburg, Haojie Cao, Sjoerd B. van der Meulen, Ana Solopova, Oscar P. Kuipers*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

11 Citations (Scopus)
471 Downloads (Pure)

Abstract

Metabolic engineering and synthetic biology approaches have prospered the field of biotechnology, in which the main focus has been on Escherichia coli and Saccharomyces cerevisiae as microbial workhorses. In more recent years, improving the Gram-positive bacteria Lactococcus lactis and Bacillus subtilis as production hosts has gained increasing attention. This review will demonstrate the different levels at which these bacteria can be engineered and their various application possibilities. For instance, engineered L. lactis strains show great promise for biomedical applications. Moreover, we provide an overview of recent synthetic biology tools that facilitate the use of these two microorganisms even more.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalCurrent Opinion in Biotechnology
Volume59
Early online date18-Feb-2019
DOIs
Publication statusPublished - Oct-2019

Keywords

  • Metabolic engineering
  • synthetic biology
  • Lactobacillus lactis
  • Bacillus subtilis
  • Cell factories
  • REGULATED EXPRESSION SYSTEM
  • GENE-EXPRESSION
  • TRANSCRIPTION MACHINERY
  • PATHWAY
  • GENOME
  • CONSTRUCTION
  • OPTIMIZATION
  • PEPTIDES
  • PROTEINS
  • NETWORK

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