Metal-dependence of xylose isomerase from Piromyces sp. E2 explored by activity profiling and protein crystallography

Misun Lee, Henriette J Rozeboom, Paul de Waal, Rene de Jong, Hanna M Dudek, Dick B Janssen

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)
273 Downloads (Pure)

Abstract

Xylose isomerase from Piromyces sp. E2 (PirXI) can be used for equipping Saccharomyces cerevisiae with the capacity to ferment xylose to ethanol. The biochemical properties and structure of the enzyme have not been described even though its metal content, catalytic parameters and expression level are critical for rapid xylose utilization. We have isolated the enzyme after high-level expression in E. coli, analyzed the metal-dependence of its catalytic properties and solved twelve crystal structures in the presence of different metals, substrates, and substrate analogs. The activity assays revealed that various bivalent metals can activate PirXI for xylose isomerization. Among these metals, Mn2+ is the most favorable for catalytic activity. Furthermore, the enzyme shows the highest affinity for Mn2+ which was established by measuring the activation constants (Kact) for different metals. Metal analysis of the purified enzyme showed that in vivo the enzyme binds a mixture of metals which is determined by metal availability as well as the affinity, indicating that native metal composition can influence activity. The crystal structures show the presence of an active site similar to that of other xylose isomerases, with a D-xylose binding site containing two tryptophans and a catalytic histidine, as well as two metal binding sites that are formed by carboxylate groups of conserved aspartates and glutamates. The binding positions and conformations of the metal-coordinating residues varied slightly for different metals, which is hypothesized to contribute to the observed metal-dependence of the isomerase activity.

Original languageEnglish
Pages (from-to)5991-6005
Number of pages15
JournalBiochemistry
Volume56
Issue number45
Early online date18-Oct-2017
DOIs
Publication statusPublished - 2017

Keywords

  • xylose isomerase
  • protein engineering
  • protein crystallography
  • metal dependence
  • catalytic activity
  • biocatalysis
  • CLOSTRIDIUM-THERMOSULFUROGENES
  • STREPTOMYCES-VIOLACEORUBER
  • ENGINEERED SACCHAROMYCES-CEREVISIAE
  • THERMOSTABLE GLUCOSE-ISOMERASE; ALDOSE-KETOSE INTERCONVERSION
  • SITE-DIRECTED MUTAGENESIS
  • MEDIATED HYDRIDE SHIFT
  • ACTINOPLANES-MISSOURIENSIS
  • FUNCTIONAL EXPRESSION
  • ETHANOL-PRODUCTION

Cite this