A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein the method comprises inactivation of at least one gene involved in methylhexahydroindanedione propionate degradation. Strains with (multiple) inactivated steroid degrading enzyme genes according to the invention can be used in the accumulation of steroid intermediates. Accumulation products are for example 3aa-H-4a (3-'propionic acid)-7ass- methylhexahydro-1,5-indanedione (HIP), 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-thene-9,17-dione (3-HSA), 1,4-androstadiene-3,17-dione (ADD) and 3aa-H-4a(3'-propionic acid)-5a-hydroxy-7ass-methylhexahydro-1 -indanone-d- lactone (HIL).
|IPC||C12P 33/ 00 A I|
|Publication status||Published - 26-Feb-2009|