Method for the production of modified steroid degrading microorganisms and there use

Robert van der Geize; Gerda I. Hessels; Lubbert Dijkhuizen

Method for the production of modified steroid degrading microorganisms and there use

Robert van der Geize (Inventor), Gerda I. Hessels (Inventor), Lubbert Dijkhuizen (Inventor)

Host-Microbe Interactions

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Abstract

A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein the method comprises inactivation of at least one gene involved in methylhexahydroindanedione propionate degradation. Strains with (multiple) inactivated steroid degrading enzyme genes according to the invention can be used in the accumulation of steroid intermediates. Accumulation products are for example 3aa-H-4a (3-'propionic acid)-7ass- methylhexahydro-1,5-indanedione (HIP), 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-thene-9,17-dione (3-HSA), 1,4-androstadiene-3,17-dione (ADD) and 3aa-H-4a(3'-propionic acid)-5a-hydroxy-7ass-methylhexahydro-1 -indanone-d- lactone (HIL).

Original language	English
Patent number	WO2009024572
IPC	C12P 33/ 00 A I
Priority date	21/08/2007
Publication status	Published - 26-Feb-2009

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title = "Method for the production of modified steroid degrading microorganisms and there use",

abstract = "A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein the method comprises inactivation of at least one gene involved in methylhexahydroindanedione propionate degradation. Strains with (multiple) inactivated steroid degrading enzyme genes according to the invention can be used in the accumulation of steroid intermediates. Accumulation products are for example 3aa-H-4a (3-'propionic acid)-7ass- methylhexahydro-1,5-indanedione (HIP), 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-thene-9,17-dione (3-HSA), 1,4-androstadiene-3,17-dione (ADD) and 3aa-H-4a(3'-propionic acid)-5a-hydroxy-7ass-methylhexahydro-1 -indanone-d- lactone (HIL).",

author = "{van der Geize}, Robert and Hessels, {Gerda I.} and Lubbert Dijkhuizen",

year = "2009",

month = feb,

day = "26",

language = "English",

type = "Patent",

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TY - PAT

T1 - Method for the production of modified steroid degrading microorganisms and there use

AU - van der Geize, Robert

AU - Hessels, Gerda I.

AU - Dijkhuizen, Lubbert

PY - 2009/2/26

Y1 - 2009/2/26

N2 - A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein the method comprises inactivation of at least one gene involved in methylhexahydroindanedione propionate degradation. Strains with (multiple) inactivated steroid degrading enzyme genes according to the invention can be used in the accumulation of steroid intermediates. Accumulation products are for example 3aa-H-4a (3-'propionic acid)-7ass- methylhexahydro-1,5-indanedione (HIP), 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-thene-9,17-dione (3-HSA), 1,4-androstadiene-3,17-dione (ADD) and 3aa-H-4a(3'-propionic acid)-5a-hydroxy-7ass-methylhexahydro-1 -indanone-d- lactone (HIL).

AB - A method is described to construct genetically modified strains of steroid degrading micro-organisms wherein the method comprises inactivation of at least one gene involved in methylhexahydroindanedione propionate degradation. Strains with (multiple) inactivated steroid degrading enzyme genes according to the invention can be used in the accumulation of steroid intermediates. Accumulation products are for example 3aa-H-4a (3-'propionic acid)-7ass- methylhexahydro-1,5-indanedione (HIP), 3-hydroxy-9,10-secoandrosta- 1,3,5(10)-thene-9,17-dione (3-HSA), 1,4-androstadiene-3,17-dione (ADD) and 3aa-H-4a(3'-propionic acid)-5a-hydroxy-7ass-methylhexahydro-1 -indanone-d- lactone (HIL).

M3 - Patent

M1 - WO2009024572

ER -