Methods to Analyze Motility in Eury- and Crenarchaea

Megha Patro, Marleen van Wolferen, Xing Ye, Sonja-Verena Albers*, Tessa E F Quax*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


Many archaea display swimming motility in liquid medium, which is empowered by the archaellum. Directional movement requires a functional archaellum and a sensing system, such as the chemotaxis system that is used by Euryarchaea. Two well-studied models are the euryarchaeon Haloferax volcanii and the crenarchaeon Sulfolobus acidocaldarius. In this chapter we describe two methods to analyze their swimming behavior and directional movement: (a) time-lapse microscopy under native temperatures and (b) spotting on semi-solid agar or gelrite plates. Whereas the first method allows for deep analysis of swimming behavior, the second method is suited for high throughput comparison of multiple strains.

Original languageEnglish
Title of host publicationArchaea
Subtitle of host publicationMethods and Protocols
EditorsSébastien Ferreira-Cerca
Number of pages13
ISBN (Electronic)978-1-0716-2445-6
ISBN (Print)978-1-0716-2444-9
Publication statusPublished - 2022

Publication series

NameMethods in molecular biology (Clifton, N.J.)
ISSN (Print)1064-3745


  • Agar
  • Archaeal Proteins
  • Chemotaxis
  • Haloferax volcanii
  • Sulfolobus acidocaldarius

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