Microfluidic dissolved oxygen gradient generator biochip as a useful tool in bacterial biofilm studies

MacIej Skolimowski, Martin Weiss Nielsen, Jenny Emnéus, Søren Molin, Rafael Taboryski, Claus Sternberg, Martin Dufva, Oliver Geschke

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A microfluidic chip for generation of gradients of dissolved oxygen was designed, fabricated and tested. The novel way of active oxygen depletion through a gas permeable membrane was applied. Numerical simulations for generation of O2gradients were correlated with measured oxygen concentrations. The developed microsystem was used to study growth patterns of the bacterium Pseudomonas aeruginosa in medium with different oxygen concentrations. The results showed that attachment of Pseudomonas aeruginosa to the substrate changed with oxygen concentration. This demonstrates that the device can be used for studies requiring controlled oxygen levels and for future studies of microaerobic and anaerobic conditions. © 2010 The Royal Society of Chemistry.
Original languageEnglish
Pages (from-to)2162-2169
Number of pages8
JournalLab on a Chip - Miniaturisation for Chemistry and Biology
Issue number16
Publication statusPublished - 21-Aug-2010
Externally publishedYes


  • dissolved oxygen
  • aerobic metabolism
  • anaerobic metabolism
  • article
  • biochip
  • biofilm
  • gas permeability
  • membrane permeability
  • microfluidics
  • nonhuman
  • oxygen concentration
  • priority journal
  • Pseudomonas aeruginosa

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