@inbook{7e1277b6faba4fb98a977fbfbe069e81,
title = "Microfluidics for single-cell study of antibiotic tolerance and persistence induced by nutrient limitation",
abstract = "Nutrient limitation is one of the most common triggers of antibiotic tolerance and persistence. Here, we present two microfluidic setups to study how spatial and temporal variation in nutrient availability lead to increased survival of bacteria to antibiotics. The first setup is designed to mimic the growth dynamics of bacteria in spatially structured populations (e.g., biofilms) and can be used to study how spatial gradients in nutrient availability, created by the collective metabolic activity of a population, increase antibiotic tolerance. The second setup captures the dynamics of feast-and-famine cycles that bacteria recurrently encounter in nature, and can be used to study how phenotypic heterogeneity in growth resumption after starvation increases survival of clonal bacterial populations. In both setups, the growth rates and metabolic activity of bacteria can be measured at the single-cell level. This is useful to build a mechanistic understanding of how spatiotemporal variation in nutrient availability triggers bacteria to enter phenotypic states that increase their tolerance to antibiotics.",
keywords = "Antibiotic persistence, Antibiotic tolerance, Biofilms, Feast-and-famine dynamics, Microfluidics, Nutrient limitation, Phenotypic heterogeneity, Single-cell measurements",
author = "Stefany Moreno-G{\'a}mez and {Dal Co}, Alma and {van Vliet}, Simon and Martin Ackermann",
note = "Funding Information: We thank Daniel J. Kiviet for developing large parts of the protocols discussed in this chapter. S.M.-G. and M.A. were supported by Eawag and ETH Zurich, and by grants nr. 31003A_149267 and 31003A_169978 from the Swiss National Science Foundation. S. M.-G. was also supported by a Starting Independent Researcher Grant 309555 of the European Research Council and a Vidi fellowship (864.11.012) of the Netherlands Organization for Scientific Research. A.D.C. is supported by Harvard University and the Materials Research Science and Engineering Center (DMR-1420570). Publisher Copyright: {\textcopyright} 2021, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1621-5_8",
language = "English",
isbn = "978-1-0716-1620-8",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "107--124",
editor = "Natalie Verstraeten and Jan Michiels",
booktitle = "Bacterial Persistence",
edition = "2",
}