Microglia replenished OHSC: A culture system to study in vivo like adult microglia

Annette Masuch, Rianne van der Pijl, Lisa Füner, Yochai Wolf, Bart Eggen, Erik Boddeke, Knut Biber*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Recent data suggest that ramified microglia fulfil various tasks in the brain. However, to investigate this unique cell type cultured primary microglia are only a poor model. We here describe a method to deplete and repopulate organotypic hippocampal slice cultures (OHSC) with ramified microglia isolated from adult mouse brain creating microglia-replenished OHSC (Mrep-OHSC). Replenished microglia integrate into the tissue and ramify to a degree indistinguishable from their counterparts in the mouse brain. Moreover, wild-type slices replenished with microglia from TNFα-deficient animals provide similar results as OHSC prepared from microglia-specific TNFα-knockout mice (CX3CR1(cre) /TNFα(fl/fl) ). Furthermore, this study demonstrates that replenished microglia in OHSC maintain original functions and properties acquired in vivo. Microglia from ERCC1(Δ/ko) mice, a mouse model of accelerated aging, maintain enhanced Mac2 expression and their activated phenotype after replenishment to wild-type OHSC tissue. Thus, the present study demonstrates that Mrep-OHSC are a unique tool to construct chimeric brain slices allowing studying the function of different phenotypes of in vivo like microglia in a tissue culture setting. GLIA 2016.

Original languageEnglish
Pages (from-to)1285-1297
Number of pages13
JournalGlia
Volume64
Issue number8
DOIs
Publication statusPublished - Aug-2016

Keywords

  • hippocampus
  • NMDA
  • neuroprotection
  • TNF alpha
  • priming
  • ERCC1
  • HIPPOCAMPAL SLICE CULTURES
  • CENTRAL-NERVOUS-SYSTEM
  • TUMOR-NECROSIS-FACTOR
  • DNA-REPAIR
  • BRAIN
  • CELLS
  • INJURY
  • MICE
  • EXCITOTOXICITY
  • NEURONS

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