Microparticle tissue factor activity is increased in cancer patients prior to the development of venous thromboembolism

A. Kleinjan, F.F. Van Doormaal, R.J. Berckmans, N. MacKman, D.A. Manly, P.W. Kamphuisen, D.J. Richel, H.R. Buller, A. Sturk, R. Nieuwland

Research output: Contribution to conferenceAbstractAcademic


Introduction: Cancer greatly increases the risk of venous thromboem-bolism (VTE). Here, we investigated the contribution of microparti-cle-dependent procoagulant activity to the prothrombotic state in these patients. Methods: In 43 cancer patients without VTE at entry and 22 healthy volunteers, markers of in vivo and microparticle-dependent coagulation were measured and patients were prospectively followed for 6 months for the development of VTE. Procoagulant activity of microparticles (MPs) was measured using a phospholipid dependent test (STA Procoag PLL), a factor Xa-generation assay (Xa-assay) with and without anti-tissue factor (TF), and a fibrin generation test (FGT) with and without anti-factor VII(a). Results: Markers of in vivo coagulation activation and total number of circulating MPs were elevated in cancer patients compared to controls (F1+2 246 vs. 156 pM, thrombin-antithrombin complexes 4.1 vs. 3.0 mg/L, D-dimer 0.76 vs. 0.22 mg/L and 5.53 x 106 vs. 3.37 x 106 microparticles per mL; all P <0.001). Five cancer patients (12%) developed VTE during follow-up. Patients with VTE had comparable levels of coagulation activation markers and phos-pholipid dependent MP procoagulant activity. However, TF-mediated Xa-generation (0.82 vs. 0.21 pg/mL, P = 0.016) and the VIIa-dependent FGT (13% vs. 0%, P = 0.036) were higher in the VTE group compared with the non-VTE group.
Original languageEnglish
Number of pages1
Publication statusPublished - 1-Jul-2011


  • thromboplastin
  • procoagulant
  • marker
  • blood clotting factor 10a
  • fibrin
  • blood clotting factor 7
  • thrombin antithrombin complex
  • D dimer
  • phospholipid
  • human
  • cancer patient
  • venous thromboembolism
  • society
  • hemostasis
  • thrombosis
  • patient
  • assay
  • follow up
  • neoplasm
  • risk
  • normal human

Cite this