Microsensors for in vivo Measurement of Glutamate in Brain Tissue

Si Qin*, Miranda van der Zeyden, Weite H. Oldenziel, Thomas I. F. H. Cremers, Ben H. C. Westerink

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

51 Citations (Scopus)
270 Downloads (Pure)

Abstract

Several immobilized enzyme-based electrochemical biosensors for glutamate detection have been developed over the last decade. In this review, we compare first and second generation sensors. Structures, working mechanisms, interference prevention, in vitro detection characteristics and in vivo performance are summarized here for those sensors that have successfully detected brain glutamate in vivo. In brief, first generation sensors have a simpler structure and are faster in glutamate detection. They also show a better sensitivity to glutamate during calibration in vitro. For second generation sensors, besides their less precise detection, their fabrication is difficult to reproduce, even with a semi-automatic dip-coater. Both generations of sensors can detect glutamate levels in vivo, but the reported basal levels are different. In general, second generation sensors detect higher basal levels of glutamate compared with the results obtained from first generation sensors. However, whether the detected glutamate is indeed from synaptic sources is an issue that needs further attention.

Original languageEnglish
Pages (from-to)6860-6884
Number of pages25
JournalSensors
Volume8
Issue number11
DOIs
Publication statusPublished - Nov-2008

Keywords

  • First generation biosensor
  • second generation biosensor
  • glutamate
  • in vivo detection
  • IMMOBILIZED GLUCOSE-OXIDASE
  • NAFION-COATED ELECTRODES
  • AGE-RELATED-CHANGES
  • AMPEROMETRIC BIOSENSOR
  • ENZYME ELECTRODE
  • ELECTROCHEMICAL CHARACTERIZATION
  • MULTISITE MICROELECTRODES
  • CONDUCTING POLYMER
  • POLYPYRROLE FILMS
  • ANESTHETIZED RAT

Cite this