Mineralization of the herbicide 2,3,6-trichlorobenzoic acid by a co-culture of anaerobic and aerobic bacteria

Jan Gerritse*, Jan C. Gottschal

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    45 Citations (Scopus)

    Abstract

    Bacteria from an anaerobic enrichment reductively removed chlorine from the ortho- position of 2,3,6-trichlorobenzoic acid (2,3,6-TBA) producing 2,5-dichlorobenzoate (2,5-DBA). The strictly aerobic bacterium Pseudomonas aeruginosa JB2 subsequently used 2,5-DBA as a growth substrate in the presence of oxygen. The anaerobic dechlorinating microbial population was grown with P. aeruginosa JB2 in continuous culture. Inside the liquid culture, a nylon netting, on a stainless-steel support, contained vermiculite particles to provide a strictly anaerobic environment within the aerated culture. Complete mineralization of 2,3,6-TBA depended on the extent of oxygen input into the reactor. Under strictly anaerobic conditions 2,5-DBA and Cl- were produced stoichiometrically through the reductive dechlorination of 2,3,6-TBA. This process of reductive dechlorination was not inhibited by (moderate) aeration resulting in an O2-concentration of 0.3-0.5-mu-M in the culture liquid.

    Original languageEnglish
    Pages (from-to)89-98
    Number of pages10
    JournalFEMS Microbial Ecology
    Volume101
    Issue number2
    DOIs
    Publication statusPublished - 1-Jul-1992

    Keywords

    • COCULTURE
    • ANAEROBIC PLUS AEROBIC BACTERIA
    • 2,3,6-TRICHLOROBENZOIC ACID
    • REDUCTIVE DECHLORINATION
    • 2,5-DICHLOROBENZOIC ACID
    • MICROAEROBIC MINERALIZATION
    • HALOGENATED AROMATIC-COMPOUNDS
    • ALCALIGENES-DENITRIFICANS
    • PSEUDOMONAS-AERUGINOSA
    • METHANOGENIC BACTERIA
    • DEGRADATION
    • DEHALOGENATION
    • SEDIMENT
    • ISOMERS
    • SOIL

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