Mitochondrial damage by α-synuclein causes cell death in human dopaminergic neurons

Goutham K Ganjam, Kathrin Bolte, Lina A Matschke, Sandra Neitemeier, Amalia M Dolga, Matthias Höllerhage, Günter U Höglinger, Agata Adamczyk, Niels Decher, Wolfgang H Oertel, Carsten Culmsee

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Abstract

Evolving concepts on Parkinson's disease (PD) pathology suggest that α-synuclein (aSYN) promote dopaminergic neuron dysfunction and death through accumulating in the mitochondria. However, the consequence of mitochondrial aSYN localisation on mitochondrial structure and bioenergetic functions in neuronal cells are poorly understood. Therefore, we investigated deleterious effects of mitochondria-targeted aSYN in differentiated human dopaminergic neurons in comparison with wild-type (WT) aSYN overexpression and corresponding EGFP (enhanced green fluorescent protein)-expressing controls. Mitochondria-targeted aSYN enhanced mitochondrial reactive oxygen species (ROS) formation, reduced ATP levels and showed severely disrupted structure and function of the dendritic neural network, preceding neuronal death. Transmission electron microscopy illustrated distorted cristae and many fragmented mitochondria in response to WT-aSYN overexpression, and a complete loss of cristae structure and massively swollen mitochondria in neurons expressing mitochondria-targeted aSYN. Further, the analysis of mitochondrial bioenergetics in differentiated dopaminergic neurons, expressing WT or mitochondria-targeted aSYN, elicited a pronounced impairment of mitochondrial respiration. In a pharmacological compound screening, we found that the pan-caspase inhibitors QVD and zVAD-FMK, and a specific caspase-1 inhibitor significantly prevented aSYN-induced cell death. In addition, the caspase inhibitor QVD preserved mitochondrial function and neuronal network activity in the human dopaminergic neurons overexpressing aSYN. Overall, our findings indicated therapeutic effects by caspase-1 inhibition despite aSYN-mediated alterations in mitochondrial morphology and function.

Original languageEnglish
Article number865
Number of pages16
JournalCell death & disease
Volume10
Issue number11
DOIs
Publication statusPublished - 14-Nov-2019

Keywords

  • PARKINSONS-DISEASE
  • IN-VITRO
  • OXIDATIVE STRESS
  • APOPTOTIC DEATH
  • COMPLEX-I
  • DYSFUNCTION
  • FERROPTOSIS
  • MECHANISMS
  • INHIBITION
  • PROTEIN

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