Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles

Anne-Marie Lambeir; Ignace Lasters; Nadir Mrabet; Wim Quax; Jan M. Van Der Laan; Onno Misset

Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles

Anne-Marie Lambeir (Inventor), Ignace Lasters (Inventor), Nadir Mrabet (Inventor), Wim Quax (Inventor), Jan M. Van Der Laan (Inventor), Onno Misset (Inventor)

Research output: Patent

Abstract

A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pKa of the metal coordinating ligands or altering the Kassfor the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.

Original language	English
Patent number	US5340738
IPC	C12R 1/ 19 A N
Priority date	04/01/1990
Publication status	Published - 23-Aug-1994

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title = "Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles",

abstract = "A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pKa of the metal coordinating ligands or altering the Kassfor the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.",

author = "Anne-Marie Lambeir and Ignace Lasters and Nadir Mrabet and Wim Quax and {Van Der Laan}, {Jan M.} and Onno Misset",

year = "1994",

month = aug,

day = "23",

language = "English",

type = "Patent",

note = "US5340738; C12R 1/ 19 A N",

}

TY - PAT

T1 - Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles

AU - Lambeir, Anne-Marie

AU - Lasters, Ignace

AU - Mrabet, Nadir

AU - Quax, Wim

AU - Van Der Laan, Jan M.

AU - Misset, Onno

PY - 1994/8/23

Y1 - 1994/8/23

N2 - A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pKa of the metal coordinating ligands or altering the Kassfor the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.

AB - A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pKa of the metal coordinating ligands or altering the Kassfor the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.

M3 - Patent

M1 - US5340738

ER -

Modified prokaryotic glucose isomerase enzymes with altered pH activity profiles

Abstract

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