Modular Approaches to Synthesize Activity- and Affinity-Based Chemical Probes

Antonie J. van der Zouwen, Martin D. Witte*

*Corresponding author for this work

Research output: Contribution to journalReview articleAcademicpeer-review

3 Citations (Scopus)
47 Downloads (Pure)


Combinatorial and modular methods to synthesize small molecule modulators of protein activity have proven to be powerful tools in the development of new drug-like molecules. Over the past decade, these methodologies have been adapted toward utilization in the development of activity- and affinity-based chemical probes, as well as in chemoproteomic profiling. In this review, we will discuss how methods like multicomponent reactions, DNA-encoded libraries, phage displays, and others provide new ways to rapidly screen novel chemical probes against proteins of interest.

Original languageEnglish
Article number644811
Number of pages17
JournalFrontiers in Chemistry
Publication statusPublished - 15-Apr-2021


  • chemical probe design
  • activity-based probe
  • affinity-based probe
  • protein profiling
  • DNA-templated
  • SuFEx
  • iminoboronate chemistry

Cite this