Modulation of Dendritic-Epithelial Cell Responses against Sphingomonas Paucimobilis by Dietary Fibers

Miriam Bermudez-Brito, Marijke M. Faas, Paul de Vos*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)
222 Downloads (Pure)

Abstract

Non-fermenting Gram-negative bacilli, such as Sphingomonas paucimobilis (S. paucimobilis), are among the most widespread causes of nosocomial infections. Up to now, no definitive guidelines exist for antimicrobial therapy for S. paucimobilis infections. As we have shown that some dietary fibers exhibit pronounced immune-regulatory properties, we hypothesized that specific immune active dietary fibers might modulate the responses against S. paucimobilis. We studied the immunomodulatory effects of dietary fibers against S. paucimobilis on cytokine release and maturation of human dendritic cells (DCs) in co-cultures of DCs and intestinal epithelial cells (IECs). S. paucimobilis infection resulted in increased release of pro-inflammatory cytokines and chemokines by DCs/IECs; these effects were strongly attenuated by specific dietary fibers. Chicory inulin, sugar beet pectin, and both starches had the strongest regulatory effects. IL-12 and TNF-alpha were drastically diminished upon exposure to chicory inulin and sugar beet pectin, or both starches. High-maize 260, was more effective in the reduction of chemokine release than the others fibers tested. In summary, chicory inulin, sugar beet pectin, High-maize 260, and Novelose 330 attenuate S. paucimobilis-induced cytokines. These results demonstrate that dietary fibers with a specific chemical composition can be used to manage immune responses against pathogens such as S. paucimobilis.

Original languageEnglish
Article number30277
Number of pages8
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - 25-Jul-2016

Keywords

  • T-CELLS
  • PROINFLAMMATORY CYTOKINES
  • INTESTINAL INFLAMMATION
  • GLYCOSPHINGOLIPIDS
  • INTERLEUKIN-10
  • INFECTIONS
  • BACTEREMIA
  • THERAPY
  • PECTINS
  • SIGNALS

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