Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli Ulcer

Nana Ama Amissah; Monika A. Chlebowicz; Anthony Ablordey; Artur J. Sabat; Caitlin S. Tetteh; Isaac Prah; Tjip S. van der Werf; Alex W. Friedrich; Jan Maarten van Dijl; John W. Rossen; Ymkje Stienstra

doi:10.1371/journal.pntd.0004049

Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli Ulcer

Nana Ama Amissah^*, Monika A. Chlebowicz, Anthony Ablordey, Artur J. Sabat, Caitlin S. Tetteh, Isaac Prah, Tjip S. van der Werf, Alex W. Friedrich, Jan Maarten van Dijl, John W. Rossen, Ymkje Stienstra

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Background

Buruli ulcer (BU) is a skin infection caused by Mycobacterium ulcerans. The wounds of most BU patients are colonized with different microorganisms, including Staphylococcus aureus.

Methodology

This study investigated possible patient-to-patient transmission events of S. aureus during wound care in a health care center. S. aureus isolates from different BU patients with overlapping visits to the clinic were whole-genome sequenced and analyzed by a gene-by-gene approach using SeqSphere(+) software. In addition, sequence data were screened for the presence of genes that conferred antibiotic resistance.

Principal Findings

SeqSphere(+) analysis of whole-genome sequence data confirmed transmission of methicillin resistant S. aureus (MRSA) and methicillin susceptible S. aureus among patients that took place during wound care. Interestingly, our sequence data show that the investigated MRSA isolates carry a novel allele of the fexB gene conferring chloramphenicol resistance, which had thus far not been observed in S. aureus.

Original language	English
Article number	0004049
Number of pages	12
Journal	PLoS Neglected Tropical Diseases
Volume	9
Issue number	9
DOIs	https://doi.org/10.1371/journal.pntd.0004049
Publication status	Published - Sept-2015

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Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli UlcerFinal publisher's version, 644 KBLicence: CC BY

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Amissah, N. A., Chlebowicz, M. A., Ablordey, A., Sabat, A. J., Tetteh, C. S., Prah, I., van der Werf, T. S., Friedrich, A. W., van Dijl, J. M., Rossen, J. W., & Stienstra, Y. (2015). Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli Ulcer. PLoS Neglected Tropical Diseases, 9(9), Article 0004049. https://doi.org/10.1371/journal.pntd.0004049

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title = "Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli Ulcer",

abstract = "BackgroundBuruli ulcer (BU) is a skin infection caused by Mycobacterium ulcerans. The wounds of most BU patients are colonized with different microorganisms, including Staphylococcus aureus.MethodologyThis study investigated possible patient-to-patient transmission events of S. aureus during wound care in a health care center. S. aureus isolates from different BU patients with overlapping visits to the clinic were whole-genome sequenced and analyzed by a gene-by-gene approach using SeqSphere(+) software. In addition, sequence data were screened for the presence of genes that conferred antibiotic resistance.Principal FindingsSeqSphere(+) analysis of whole-genome sequence data confirmed transmission of methicillin resistant S. aureus (MRSA) and methicillin susceptible S. aureus among patients that took place during wound care. Interestingly, our sequence data show that the investigated MRSA isolates carry a novel allele of the fexB gene conferring chloramphenicol resistance, which had thus far not been observed in S. aureus.",

author = "Amissah, {Nana Ama} and Chlebowicz, {Monika A.} and Anthony Ablordey and Sabat, {Artur J.} and Tetteh, {Caitlin S.} and Isaac Prah and {van der Werf}, {Tjip S.} and Friedrich, {Alex W.} and {van Dijl}, {Jan Maarten} and Rossen, {John W.} and Ymkje Stienstra",

year = "2015",

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journal = "PLoS Neglected Tropical Diseases",

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AU - Amissah, Nana Ama

AU - Chlebowicz, Monika A.

AU - Ablordey, Anthony

AU - Sabat, Artur J.

AU - Tetteh, Caitlin S.

AU - Prah, Isaac

AU - van der Werf, Tjip S.

AU - Friedrich, Alex W.

AU - van Dijl, Jan Maarten

AU - Rossen, John W.

AU - Stienstra, Ymkje

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N2 - BackgroundBuruli ulcer (BU) is a skin infection caused by Mycobacterium ulcerans. The wounds of most BU patients are colonized with different microorganisms, including Staphylococcus aureus.MethodologyThis study investigated possible patient-to-patient transmission events of S. aureus during wound care in a health care center. S. aureus isolates from different BU patients with overlapping visits to the clinic were whole-genome sequenced and analyzed by a gene-by-gene approach using SeqSphere(+) software. In addition, sequence data were screened for the presence of genes that conferred antibiotic resistance.Principal FindingsSeqSphere(+) analysis of whole-genome sequence data confirmed transmission of methicillin resistant S. aureus (MRSA) and methicillin susceptible S. aureus among patients that took place during wound care. Interestingly, our sequence data show that the investigated MRSA isolates carry a novel allele of the fexB gene conferring chloramphenicol resistance, which had thus far not been observed in S. aureus.

AB - BackgroundBuruli ulcer (BU) is a skin infection caused by Mycobacterium ulcerans. The wounds of most BU patients are colonized with different microorganisms, including Staphylococcus aureus.MethodologyThis study investigated possible patient-to-patient transmission events of S. aureus during wound care in a health care center. S. aureus isolates from different BU patients with overlapping visits to the clinic were whole-genome sequenced and analyzed by a gene-by-gene approach using SeqSphere(+) software. In addition, sequence data were screened for the presence of genes that conferred antibiotic resistance.Principal FindingsSeqSphere(+) analysis of whole-genome sequence data confirmed transmission of methicillin resistant S. aureus (MRSA) and methicillin susceptible S. aureus among patients that took place during wound care. Interestingly, our sequence data show that the investigated MRSA isolates carry a novel allele of the fexB gene conferring chloramphenicol resistance, which had thus far not been observed in S. aureus.

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Molecular Characterization of Staphylococcus aureus Isolates Transmitted between Patients with Buruli Ulcer

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