Mouse Lung Tissue Slice Culture

Xinhui Wu, Eline M van Dijk, I Sophie T Bos, Loes E M Kistemaker, Reinoud Gosens

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

2 Citations (Scopus)

Abstract

Precision-cut lung slices (PCLS) represent an ex vivo model widely used in visualizing interactions between lung structure and function. The major advantage of this technique is that the presence, differentiation state, and localization of the more than 40 cell types that make up the lung are in accordance with the physiological situation found in lung tissue, including the right localization and patterning of extracellular matrix elements. Here we describe the methodology involved in preparing and culturing PCLS followed by detailed practical information about their possible applications.

Original languageEnglish
Title of host publicationMouse cell culture
Place of PublicationNew York
PublisherHumana Press
Pages297-311
Number of pages15
ISBN (Electronic)978-1-4939-9086-3
ISBN (Print)978-1-4939-9085-6
DOIs
Publication statusPublished - 21-Feb-2019

Publication series

NameMethods in Molecular Biology
Volume1940
ISSN (Print)1064-3745

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