mScarlet3: a brilliant and fast-maturing red fluorescent protein

Theodorus W.J. Gadella*, Laura van Weeren, Jente Stouthamer, Mark A. Hink, Anouk H.G. Wolters, Ben N.G. Giepmans, Sylvain Aumonier, Jérôme Dupuy, Antoine Royant

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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We report the evolution of mScarlet3, a cysteine-free monomeric red fluorescent protein with fast and complete maturation, as well as record brightness, quantum yield (75%) and fluorescence lifetime (4.0 ns). The mScarlet3 crystal structure reveals a barrel rigidified at one of its heads by a large hydrophobic patch of internal residues. mScarlet3 behaves well as a fusion tag, displays no apparent cytotoxicity and it surpasses existing red fluorescent proteins as a Förster resonance energy transfer acceptor and as a reporter in transient expression systems.

Original languageEnglish
Pages (from-to)541-545
Number of pages5
JournalNature Methods
Issue number4
Early online date27-Mar-2023
Publication statusPublished - Apr-2023

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