Multi-color electron microscopy by element-guided identification of cells, organelles and molecules

Marijke Scotuzzi, Jeroen Kuipers, Dasha I. Wensveen, Pascal de Boer, Kees (C. ) W. Hagen, Jacob P. Hoogenboom, Ben N. G. Giepmans

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)
377 Downloads (Pure)

Abstract

Cellular complexity is unraveled at nanometer resolution using electron microscopy (EM), but interpretation of macromolecular functionality is hampered by the difficulty in interpreting greyscale images and the unidentified molecular content. We perform large-scale EM on mammalian tissue complemented with energy-dispersive X-ray analysis (EDX) to allow EM-data analysis based on elemental composition. Endogenous elements, labels (gold and cadmium-based nanoparticles) as well as stains are analyzed at ultrastructural resolution. This provides a wide palette of colors to paint the traditional grey-scale EM images for composition-based interpretation. Our proof-ofprinciple application of EM-EDX reveals that endocrine and exocrine vesicles exist in single cells in Islets of Langerhans. This highlights how elemental mapping reveals unbiased biomedical relevant information. Broad application of EM-EDX will further allow experimental analysis on large-scale tissue using endogenous elements, multiple stains, and multiple markers and thus brings nanometer-scale 'color-EM' as a promising tool to unravel molecular (de) regulation in biomedicine.

Original languageEnglish
Article number45970
Number of pages8
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 7-Apr-2017

Keywords

  • X-RAY-MICROANALYSIS
  • TRANSMISSION ELECTRON
  • BIOLOGICAL SCIENCES
  • EXOCRINE PANCREAS
  • CORRELATED LIGHT
  • QUANTUM DOTS
  • ENERGY-LOSS
  • BETA-CELLS
  • FLUORESCENCE
  • PATHOGENESIS

Cite this