Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma

Pepijn Beekman*, Agustin Enciso-Martinez, Melissa Piontek, Leon Terstappen, Wouter Roos, Cees Otto, Séverine Le Gac

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionAcademicpeer-review

Abstract

Extracellular vesicles have emerged in recent years as highly promising for understanding cell communication, drug delivery, and medical applications. Specifically, tumor-derived extracellular vesicles (tdEVs) have demonstrated excellent prognostic value in cancer diagnostics compared to imaging approaches. Despite the growing body of expertise regarding EVs, great challenges remain, notably in their handling and characterization. In complex media, other particles with similar characteristics may occlude measurements. Here, a platform is presented for the immunocapturing of tdEVs for identifying their origin followed by further multi-modal analysis by Raman spectroscopy, confocal microscopy and atomic force microscopy (AFM).

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages404-405
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: 4-Oct-20209-Oct-2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period04/10/202009/10/2020

Keywords

  • Cancer diagnostics
  • Extracellular vesicles
  • Multi-modal analysis
  • Surface modification
  • TdEVs

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