Mutational analysis of primary alcohol metabolism in the methylotrophic actinomycete Amycolatopsis methanolica

Harm J. Hektor, Lubbert Dijkhuizen

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Abstract

Mutants of the methylotrophic actinomycete Amycolatopsis methanolica unable to grow on methanol as carbon source were isolated and characterized. Mutants specifically affected in methanol utilization were deficient in formaldehyde assimilation. Mutants blocked in the first step of primary alcohol oxidation (C1-C4) had lost activity of the tetrazolium-dependent alcohol dehydrogenase, a three-component enzyme complex. This complex, or individual components, thus play a crucial role in utilization of primary alcohols in A. methanolica.

Original languageEnglish
Pages (from-to)73 - 79
Number of pages7
JournalFEMS Microbiology Letters
Volume144
Issue number1
DOIs
Publication statusPublished - 15-Oct-1996

Keywords

  • actinomycete
  • Amycolatopsis methanolica
  • primary alcohol
  • ethanol
  • methanol
  • mutant
  • mutagenesis
  • GRAM-POSITIVE BACTERIA
  • KEY ENZYME
  • DEHYDROGENASE
  • PURIFICATION
  • OXIDOREDUCTASES
  • BACILLUS
  • GROWTH

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