Using high performance liquid chromatography with electrochemical detection we determined free dopamine, noradrenaline, adrenaline and N-methyladrenaline in: (1) urines from newborns (n=32), children (n=45) and adults (n=19) and (2) adrenals, organ of Zuckerkandl, dorsal roots and perirenal brown adipose tissue from deceased fetuses (n=2), very premature (n=6) and term (n=2) newborns and infants (n=2). Data from children and adults showed that contributions of adrenaline and N-methyladrenaline to the sum of urinary free catecholamines increase with age, Relative amounts of adrenaline and N-methyladrenaline increased in both adrenal and extra adrenal chromaffin tissues from late gestation up to several months of postnatal life. Increase of adrenal N-methyladrenaline content follows endocrine maturation of the medulla, phenylethanolamine-N-methyltransferase induction and subsequent adrenaline synthesis. Relative amounts of N-methyladrenaline in extra adrenal chromaffin tissue increase in a period that is associated with its regression. Further investigations are necessary to elucidate the function and possible clinical chemical usefulness of N-methyladrenaline.
|Number of pages||14|
|Journal||Clinica chimica acta|
|Publication status||Published - 14-Jul-1995|
- EXTRA ADRENAL CHROMAFFIN TISSUE
- HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
- AMPEROMETRIC DETECTION
- EPINEPHRINE SYNTHESIS