Na+/H+ antiport activity in tonoplast vesicles from roots of the salt‐tolerant Plantago maritima and the salt‐sensitive Plantago media

M Staal*, F.J.M Maathuis, J.T.M Elzenga, J.H.M Overbeek, H.B.A Prins

*Corresponding author for this work

    Research output: Contribution to journalArticleAcademicpeer-review

    98 Citations (Scopus)

    Abstract

    Plantago species differ in their strategy towards salt stress, a major difference being the uptake and distribution of Na+ ions. A salt-sensitive (Plantago media L.) and a salt-tolerant (P. maritima L.) species were compared with respect to Na+/H+ antiport activities at the tonoplast. After exposure of the plants to 50 mM NaCl for 6 days isolated tonoplast vesicles of P. maritima showed Na+/H+ antiport activity with saturation kinetics and a K(m) of 2.4 mM Na+ . NaCl-grown P. media and the control plants of both species showed no antiport activity. Selectivity of the antiport system for Na+ was high and was determined by adding different chloride salts after formation of a DELTA-pH in the vesicles. Specific tonoplast ATPase activities were similar in the two species and did not alter after exposure to NaCl stress.

    Original languageEnglish
    Pages (from-to)179-184
    Number of pages6
    JournalPhysiologia Plantarum
    Volume82
    Issue number2
    DOIs
    Publication statusPublished - Jun-1991

    Keywords

    • PLANTAGO-MARITIMA
    • PLANTAGO-MEDIA
    • PROTON-SODIUM ANTIPORT
    • SALT TOLERANCE
    • TONOPLAST
    • H+-TRANSLOCATING ATPASE
    • MEMBRANE-VESICLES
    • PLASMA-MEMBRANE
    • RICCIA-FLUITANS
    • BARLEY
    • SODIUM
    • PYROPHOSPHATASE
    • PURIFICATION
    • HALOPHYTES
    • VANADATE

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