Abstract
The present disclosure relates to systems and methods for analysis of proteins, more in particular to nanopore systems, devices and methods for single- molecule protein analysis and sequencing. Provided is a method for translocating a target protein through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising: (a) allowing a protein translocase in solution to capture and form a complex with the target protein to be translocated; (b) contacting the translocase-target protein complex with the cis side of the nanopore and allowing for translocation of the target protein to the trans side; wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a large net ionic current flow cis-to-trans relative to the total ionic current flow, so that the target protein is captured in the nanopore with on top of the nanopore the translocase controlling the translocation.
| Original language | English |
|---|---|
| Patent number | WO2024091124 |
| Priority date | 28/10/2022 |
| Filing date | 30/10/2023 |
| Publication status | Published - 2-May-2024 |