Abstract
The invention relates to the field of genetically engineered nanopores and the use thereof in analyzing biopolymers and other (biological) compounds. Provided is a proteinaceous nanopore comprising a mutant pore-forming toxin, or a pore-forming fragment thereof, wherein the lumen-facing recognition region of the pore-forming protein or fragment thereof comprises one or more substitution(s) of lumen-facing amino acid(s) in the recognition region corresponding to amino acids 10-20 of Fragaceatoxin C (FraC), to a natural or non-natural aromatic amino acid residue.
| Original language | English |
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| Patent number | WO2022245209 |
| Priority date | 18/05/2021 |
| Filing date | 18/05/2022 |
| Publication status | Published - 24-Nov-2022 |