Nanopore spectrometry for the detection of proteins and their modifications

Roderick Versloot

doi:10.33612/diss.568483425

Nanopore spectrometry for the detection of proteins and their modifications

Roderick Versloot

Chemical Biology 1

Research output: Thesis › Thesis fully internal (DIV)

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Abstract

In this thesis, we explore the adaptability of biological nanopores towards the detection of proteins and their modifications. First, beta-barrel nanopores are modified to increase the retention and discrimantion of proteogenic peptides. An acidic-aromatic sensing region allowed the capture and detection of a mixture of peptides in the nanopore at low pH. These acidic-aromatic nanopores are then used to detect protein glycosylation, peptide chirality and isobaric peptide modifications.

Original language	English
Qualification	Doctor of Philosophy
Awarding Institution	University of Groningen
Supervisors/Advisors	Maglia, Giovanni, Supervisor Wloka, Carsten, Co-supervisor
Award date	7-Mar-2023
Place of Publication	[Groningen]
Publisher	University of Groningen
DOIs	https://doi.org/10.33612/diss.568483425
Publication status	Published - 2023

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10.33612/diss.568483425

Title and contentsFinal publisher's version, 348 KB
Chapter 1Final publisher's version, 1.18 MB
Chapter 2Final publisher's version, 2.57 MB
Chapter 3Final publisher's version, 3.14 MB
Chapter 4Final publisher's version, 2.09 MB
Chapter 5Final publisher's version, 261 KB
Chapter 6Final publisher's version, 157 KB
Chapter 7Final publisher's version, 158 KB
Chapter 8Final publisher's version, 154 KB
Chapter 9Final publisher's version, 150 KB
Complete thesisFinal publisher's version, 8.22 MB
PropositionsFinal publisher's version, 100 KB

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title = "Nanopore spectrometry for the detection of proteins and their modifications",

abstract = "In this thesis, we explore the adaptability of biological nanopores towards the detection of proteins and their modifications. First, beta-barrel nanopores are modified to increase the retention and discrimantion of proteogenic peptides. An acidic-aromatic sensing region allowed the capture and detection of a mixture of peptides in the nanopore at low pH. These acidic-aromatic nanopores are then used to detect protein glycosylation, peptide chirality and isobaric peptide modifications.",

author = "Roderick Versloot",

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language = "English",

publisher = "University of Groningen",

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T1 - Nanopore spectrometry for the detection of proteins and their modifications

AU - Versloot, Roderick

PY - 2023

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AB - In this thesis, we explore the adaptability of biological nanopores towards the detection of proteins and their modifications. First, beta-barrel nanopores are modified to increase the retention and discrimantion of proteogenic peptides. An acidic-aromatic sensing region allowed the capture and detection of a mixture of peptides in the nanopore at low pH. These acidic-aromatic nanopores are then used to detect protein glycosylation, peptide chirality and isobaric peptide modifications.

U2 - 10.33612/diss.568483425

DO - 10.33612/diss.568483425

M3 - Thesis fully internal (DIV)

PB - University of Groningen

CY - [Groningen]

ER -

Nanopore spectrometry for the detection of proteins and their modifications

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