Nanopore spectrometry for the detection of proteins and their modifications

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In this thesis, we explore the adaptability of biological nanopores towards the detection of proteins and their modifications. First, beta-barrel nanopores are modified to increase the retention and discrimantion of proteogenic peptides. An acidic-aromatic sensing region allowed the capture and detection of a mixture of peptides in the nanopore at low pH. These acidic-aromatic nanopores are then used to detect protein glycosylation, peptide chirality and isobaric peptide modifications.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • University of Groningen
  • Maglia, Giovanni, Supervisor
  • Wloka, Carsten, Co-supervisor
Award date7-Mar-2023
Place of Publication[Groningen]
Publication statusPublished - 2023

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