Abstract
The invention relates to means and methods for analysis of target analytes using nanopore-based sensors, more in particular to methods, nanopore systems and devices for single-molecule profiling of polymers, e.g. polypeptide or polysaccharides. Provided is a method for translocating a non-nucleic acid based polymer analyte through a nanopore, the nanopore being comprised in a membrane separating a fluidic chamber of a nanopore system into a cis side and a trans side, comprising adding the analyte to the cis side of and allowing for translocation, wherein the nanopore system has a cis to trans electro-osmotic force (EOF) resulting from a net ionic current flow cis to trans, preferably wherein the cis to trans EOF results from a net ionic current flow cis to trans over total ionic current flow of greater than 0.2 or less than -0.2.
| Original language | English |
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| Patent number | WO2024091123 |
| Priority date | 28/10/2022 |
| Filing date | 30/10/2023 |
| Publication status | Published - 2-May-2024 |