Near-Edge X-ray Absorption Mass Spectrometry of a Gas-Phase Peptide

Olmo Gonzalez Magana, G. Reitsma, M. Tiemens, L. Boschman, R. Hoekstra, Thomas Schlathölter

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29 Citations (Scopus)

Abstract

We have studied the dissociation of the gas-phase protonated peptide leucine enkephalin [YGGFL+H](+) upon X-ray absorption in the region of the C K-edge. The yield of photodissociation products was recorded as a function of photon energy. The total photoabsorption yield is qualitatively similar to near-edge X-ray absorption fine structure (NEXAFS) spectra recorded from condensed phase peptides and proteins. Fragment specificity reveals distinct quantitative differences between spectra obtained for different masses. Fragmentation channels can be assigned to specific electronic transitions some of which are site specific. For instance, C 1s -> pi(star) excitations in the leucine enkephalin aromatic side chains lead to relatively little fragmentation, whereas such excitations along the peptide backbone induce strong fragmentation.

Original languageEnglish
Pages (from-to)10745-10751
Number of pages7
JournalThe Journal of Physical Chemistry A
Volume116
Issue number44
DOIs
Publication statusPublished - 8-Nov-2012

Keywords

  • INDUCED DISSOCIATION
  • PROTONATED PEPTIDES
  • LEUCINE-ENKEPHALIN
  • AMINO-ACIDS
  • SPECTROSCOPY
  • PHOTOFRAGMENTATION
  • PHOTOIONIZATION
  • FRAGMENTATION
  • CONFORMATION
  • BIOMOLECULES

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