Neodymium as an alternative contrast for uranium in electron microscopy

Jeroen Kuipers; Ben N G Giepmans

doi:10.1007/s00418-020-01846-0

Neodymium as an alternative contrast for uranium in electron microscopy

Jeroen Kuipers
, Ben N G Giepmans^*

^*Corresponding author for this work

Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE)

Research output: Contribution to journal › Article › Academic › peer-review

36 Citations (Scopus)

228 Downloads (Pure)

Abstract

Uranyl acetate is the standard contrasting agent in electron microscopy (EM), but it is toxic and radioactive. We reasoned neodymium acetate might substitute uranyl acetate as a contrasting agent, and we find that neodymium acetate indeed can replace uranyl acetate in several routine applications. Since neodymium acetate is not toxic, not radioactive and easy to use, we foresee neodymium will replace uranyl in many EM sample preparation applications worldwide.

Original language	English
Pages (from-to)	271-277
Number of pages	7
Journal	Histochemistry and cell biology
Volume	153
Issue number	4
DOIs	https://doi.org/10.1007/s00418-020-01846-0
Publication status	Published - Apr-2020

Keywords

Contrast
Electron microscopy
Neodymium
Alternative contrast
Neodymium acetate
Uranyl acetate
OOLONG TEA EXTRACT
URANYL ACETATE
ULTRATHIN SECTIONS
TISSUE SECTIONS
SUBSTITUTE
LEAD

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title = "Neodymium as an alternative contrast for uranium in electron microscopy",

abstract = "Uranyl acetate is the standard contrasting agent in electron microscopy (EM), but it is toxic and radioactive. We reasoned neodymium acetate might substitute uranyl acetate as a contrasting agent, and we find that neodymium acetate indeed can replace uranyl acetate in several routine applications. Since neodymium acetate is not toxic, not radioactive and easy to use, we foresee neodymium will replace uranyl in many EM sample preparation applications worldwide.",

keywords = "Contrast, Electron microscopy, Neodymium, Alternative contrast, Neodymium acetate, Uranyl acetate, OOLONG TEA EXTRACT, URANYL ACETATE, ULTRATHIN SECTIONS, TISSUE SECTIONS, SUBSTITUTE, LEAD",

author = "Jeroen Kuipers and Giepmans, \{Ben N G\}",

year = "2020",

month = apr,

doi = "10.1007/s00418-020-01846-0",

language = "English",

volume = "153",

pages = "271--277",

journal = "Histochemistry and cell biology",

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T1 - Neodymium as an alternative contrast for uranium in electron microscopy

AU - Kuipers, Jeroen

AU - Giepmans, Ben N G

PY - 2020/4

Y1 - 2020/4

N2 - Uranyl acetate is the standard contrasting agent in electron microscopy (EM), but it is toxic and radioactive. We reasoned neodymium acetate might substitute uranyl acetate as a contrasting agent, and we find that neodymium acetate indeed can replace uranyl acetate in several routine applications. Since neodymium acetate is not toxic, not radioactive and easy to use, we foresee neodymium will replace uranyl in many EM sample preparation applications worldwide.

AB - Uranyl acetate is the standard contrasting agent in electron microscopy (EM), but it is toxic and radioactive. We reasoned neodymium acetate might substitute uranyl acetate as a contrasting agent, and we find that neodymium acetate indeed can replace uranyl acetate in several routine applications. Since neodymium acetate is not toxic, not radioactive and easy to use, we foresee neodymium will replace uranyl in many EM sample preparation applications worldwide.

KW - Contrast

KW - Electron microscopy

KW - Neodymium

KW - Alternative contrast

KW - Neodymium acetate

KW - Uranyl acetate

KW - OOLONG TEA EXTRACT

KW - URANYL ACETATE

KW - ULTRATHIN SECTIONS

KW - TISSUE SECTIONS

KW - SUBSTITUTE

KW - LEAD

U2 - 10.1007/s00418-020-01846-0

DO - 10.1007/s00418-020-01846-0

M3 - Article

C2 - 32008069

SN - 0948-6143

VL - 153

SP - 271

EP - 277

JO - Histochemistry and cell biology

JF - Histochemistry and cell biology

IS - 4

ER -

Neodymium as an alternative contrast for uranium in electron microscopy

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Keywords

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