NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma

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Abstract

MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis.

Original languageEnglish
Title of host publicationLymphoma
EditorsRalf Küppers
PublisherSpringer
Pages269-282
Number of pages14
Volume1956
ISBN (Electronic)978-1-4939-9151-8
ISBN (Print)978-1-4939-9150-1
DOIs
Publication statusE-pub ahead of print - 19-Feb-2019

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

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