@inbook{0fb96b19e9ce4554b83b0afb00b094f4,
title = "NGS-Based High-Throughput Screen to Identify MicroRNAs Regulating Growth of B-Cell Lymphoma",
abstract = "MicroRNAs (miRNAs) play important roles in development, differentiation, and homeostasis by regulating protein translation. In B-cell lymphoma, many miRNAs have altered expression levels, and for a limited subset of them, experimental data supports their functional relevance in lymphoma pathogenesis. This chapter describes an unbiased next-generation sequencing (NGS)-based high-throughput screening approach to identify miRNAs that are involved in the control of cell growth. First, we provide a protocol for performing high-throughput screening for miRNA inhibition and overexpression. Second, we describe the procedure for next-generation sequencing library preparation. Third, we provide a workflow for data analysis.",
author = "Joost Kluiver and Fubiao Niu and Ye Yuan and Klaas Kok and {van den Berg}, Anke and Agnieszka Dzikiewicz-Krawczyk",
year = "2019",
doi = "10.1007/978-1-4939-9151-8_12",
language = "English",
isbn = "978-1-4939-9150-1",
volume = "1956",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "269--282",
editor = "K{\"u}ppers, {Ralf }",
booktitle = "Lymphoma",
}