Nitric oxide measured with single-breath and tidal-breathing methods in asthma and COPD

[No Value] Rutgers, RJ Meijer*, HAM Kerstjens, TW van der Mark, GH Koeter, DS Postma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

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Nitric oxide (NO) can be measured in exhaled air with the single-breath (SB) and tidal-breathing (TB) methods, To allow comparison between different laboratories, a European Respiratory Society task force recently reported guidelines for standardization of both methods. To facilitate comparison between laboratories further, this study investigated whether there is a difference between NO values measured with SE and TB methods in subjects with asthma or chronic obstructive pulmonary disease (COPD), and in healthy subjects. Moreover, the differences between groups were studied and the influence of smoking in asthma was assessed.

Sixteen atopic nonsmoking asthmatics, 16 atopic currently smoking asthmatics, 16 nonatopic nonsmoking healthy controls, 16 nonatopic exsmokers with COPD and 16 nonatopic exsmoking healthy controls were studied.

NO concentrations differed substantially between both methods. Mean NO concentrations were higher with the SE than with the TB method in nonsmoking and in smoking asthmatics and especially so with the higher NO values. Furthermore, NO values with both methods were higher in nonsmoking asthmatics than in nonsmoking healthy subjects, NO was not significantly different between exsmokers with COPD and healthy exsmokers,

In conclusion nitric oxide values of the single-breath and tidal-breathing methods are not interchangeable. Both methods can be used to measure differences between groups.

Original languageEnglish
Pages (from-to)816-819
Number of pages4
JournalEuropean Respiratory Journal
Issue number4
Publication statusPublished - Oct-1998


  • asthma
  • chemiluminescence
  • chronic obstructive pulmonary disease
  • exhaled air
  • nitric oxide
  • smoking

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