Nitrogen and boron-doped reduced graphene oxide chemiresistive sensor for real-time monitoring dissolved oxygen in biological processes

Selvaraj Chinnathambi*, Sumit Kumar, Gert Jan Willem Euverink

*Corresponding author for this work

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We developed a nitrogen and boron-doped reduced graphene oxide (N, B-HRGO) based chemiresistive sensor to measure dissolved oxygen (DO) in a complex biological medium. The N, B-HRGO modified interdigitated micro electrode arrays (IDE) constructed as a chemiresistor by the drop-cast method. A silicon based fluorinated oxygen permeable membrane protects the surface from the interference and provides a specificity to the sensor. The sensor responded to the DO concentration changes due to modulated surface charge carrier concentration by the adsorbed dissolved oxygen molecule (Oad). For DO concentration range 0–5 mg.L−1 there was nearly 80% change in response for the sensor with membrane. The resistance of the N, B-HRGO film was measured at different DO concentrations in KNO3 solution and during the growth of Amycalotopsis methanolica bacterial fermentation. The study showed that the sensor is sensitive to the oxygen present in the solution and can detect DO consumption in a complex fermentation medium. The effect of water and the electrolyte salt ions present in the electrolyte was studied in detail. It was observed that the adsorption of water molecule increases the sensor resistance, whereas the salt ions have negligible effect on the sensor response. Because of the simple electrode structure, this chemiresistive sensor can measure DO in the micro bioreactors with a volume of few microliters. The N, B-HRGO chemiresistive sensor can also be used for DO measurement in other bioprocess applications.

Original languageEnglish
Article number100128
Number of pages4
JournalSensors and Actuators Reports
Publication statusPublished - Nov-2022


  • Adsorption and desorption
  • Chemiresistive sensor
  • Dissolved oxygen
  • Interdigitated micro electrode arrays
  • Nitrogen and Boron doping
  • Reduced graphene oxide

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