Abstract
A new procedure for site-directed mutagenesis has been applied to the shooting and rooting loci of T-DNA of an octopine Ti-plasmid of Agrobacterium tumefaciens. Mutants have been obtained which induced tumours that either developed shoots or produced more roots than normally observed. Double mutations, in which both types of T-DNA loci were affected, resulted in non-oncogenic strains. Indications have been obtained, showing that T-DNA coded oncogenic functions can be eliminated without affecting T-DNA transfer into plant cells.
Original language | English |
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Number of pages | 9 |
Journal | Plant Molecular Biology |
Volume | 2 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1983 |
Keywords
- T-DNA transfer
- non-oncogenic T-region mutants
- site-directed mutagenesis
- Agrobacterium tumefaciens