TY - JOUR
T1 - Nosocomial transmission of tet(x3), blaNDM-1 and blaOXA-97-carrying Acinetobacter baumannii conferring resistance to eravacycline and omadacycline, the Netherlands, March to August 2021
AU - Talsma, Ditmer T.
AU - Monteiro, Rodrigo
AU - del Carmen Flores-Vallejo, Rosario
AU - Heuvelmans, Maarten
AU - Le, Thuy Nga
AU - Hendrickx, Antoni P.A.
AU - Rosema, Sigrid
AU - Maat, Ianthe
AU - van Dijl, Jan Maarten
AU - Bathoorn, Erik
N1 - Publisher Copyright:
© 2024 European Centre for Disease Prevention and Control (ECDC). All rights reserved.
PY - 2024/7/1
Y1 - 2024/7/1
N2 - Carbapenem-resistant Acinetobacter baumannii (CRAb) is an important pathogen causing serious nosocomial infections. We describe an outbreak of CRAb in an intensive care unit in the Netherlands in 2021. During an outbreak of non-resistant A. baumannii, while infection control measures were in place, CRAb isolates carrying highly similar blaNDM-1- and tet(x3)-encoding plasmids were isolated from three patients over a period of several months. The chromosomal and plasmid sequences of the CRAb and non-carbapenemase-carrying A. baumannii isolates cultured from patient materials were analysed using hybrid assemblies of short-read and long-read sequences. The CRAb isolates revealed that the CRAb outbreak consisted of two different strains, carrying similar plasmids. The plasmids contained multiple antibiotic resistance genes including the tetracycline resistance gene tet(x3), and the blaNDM-1 and blaOXA-97 carbapenemase genes. We determined minimal inhibitory concentrations (MICs) for 13 antibiotics, including the newly registered tetracycline antibiotics eravacycline and omadacycline. The CRAb isolates showed high MICs for tetracycline antibiotics including eravacycline and omadacycline, except for minocycline which had a low MIC. In this study we show the value of sequencing multidrug-resistant A. baumannii for outbreak tracking and guiding outbreak mitigation measures.
AB - Carbapenem-resistant Acinetobacter baumannii (CRAb) is an important pathogen causing serious nosocomial infections. We describe an outbreak of CRAb in an intensive care unit in the Netherlands in 2021. During an outbreak of non-resistant A. baumannii, while infection control measures were in place, CRAb isolates carrying highly similar blaNDM-1- and tet(x3)-encoding plasmids were isolated from three patients over a period of several months. The chromosomal and plasmid sequences of the CRAb and non-carbapenemase-carrying A. baumannii isolates cultured from patient materials were analysed using hybrid assemblies of short-read and long-read sequences. The CRAb isolates revealed that the CRAb outbreak consisted of two different strains, carrying similar plasmids. The plasmids contained multiple antibiotic resistance genes including the tetracycline resistance gene tet(x3), and the blaNDM-1 and blaOXA-97 carbapenemase genes. We determined minimal inhibitory concentrations (MICs) for 13 antibiotics, including the newly registered tetracycline antibiotics eravacycline and omadacycline. The CRAb isolates showed high MICs for tetracycline antibiotics including eravacycline and omadacycline, except for minocycline which had a low MIC. In this study we show the value of sequencing multidrug-resistant A. baumannii for outbreak tracking and guiding outbreak mitigation measures.
UR - http://www.scopus.com/inward/record.url?scp=85198251002&partnerID=8YFLogxK
U2 - 10.2807/1560-7917.ES.2024.29.28.2400019
DO - 10.2807/1560-7917.ES.2024.29.28.2400019
M3 - Article
C2 - 38994602
AN - SCOPUS:85198251002
SN - 1025-496X
VL - 29
JO - Eurosurveillance
JF - Eurosurveillance
IS - 28
M1 - e2400019
ER -