Novel Activity-Based Probes for Broad-Spectrum Profiling of Retaining β-Exoglucosidases In Situ and In Vivo

Wouter W. Kallemeijn, Kah-Yee Li, Martin D. Witte, André R.A. Marques, Jan Aten, Saskia Scheij, Jianbing Jiang, Lianne I. Willems, Tineke M. Voorn-Brouwer, Cindy P.A.A. van Roomen, Roelof Ottenhoff, Rolf G. Boot, Hans van den Elst, Marthe T.C. Walvoort, Bogdan I. Florea, Jeroen D.C. Codée, Gijsbert A. van der Marel, Johannes M.F.G. Aerts, Herman S. Overkleeft

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Abstract

Cyclophellitol aziridine-type activity-based probes allow for ultra-sensitive visualization of mammalian β-glucosidases (GBA1, GBA2, GBA3, and LPH) as well as several non-mammalian β-glucosidases. These probes offer new ways to study β-exoglucosidases, and configurational isomers of the cyclophellitol aziridine core may give activity-based probes targeting other retaining glycosidase families.
Original languageEnglish
Pages (from-to)12529-12533
Number of pages5
JournalAngewandte Chemie International Edition
Volume51
Issue number50
DOIs
Publication statusPublished - 2012

Keywords

  • proteomics
  • glycosidases
  • glucocerebrosidase
  • fluorescent probes
  • activity-based probes

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