Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

Alejandro Mata-Cabana; Olga Sin; Renée I Seinstra; Ellen A A Nollen

doi:10.21769/BioProtoc.3053

Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

Alejandro Mata-Cabana, Olga Sin, Renée I Seinstra, Ellen A A Nollen

Research output: Contribution to journal › Article › Academic › peer-review

Abstract

C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.

Original language	English
Article number	3053
Number of pages	11
Journal	JMIR research protocols
Volume	8
Issue number	20
DOIs	https://doi.org/10.21769/BioProtoc.3053
Publication status	Published - 20-Oct-2018

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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6245569Licence: Unspecified

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title = "Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans",

abstract = "C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.",

author = "Alejandro Mata-Cabana and Olga Sin and Seinstra, {Ren{\'e}e I} and Nollen, {Ellen A A}",

year = "2018",

month = oct,

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T1 - Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

AU - Mata-Cabana, Alejandro

AU - Sin, Olga

AU - Seinstra, Renée I

AU - Nollen, Ellen A A

PY - 2018/10/20

Y1 - 2018/10/20

N2 - C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.

AB - C. elegans is widely used to investigate biological processes related to health and disease. To study protein localization, fluorescently-tagged proteins can be used in vivo or immunohistochemistry can be performed in whole worms. Here, we describe a technique to localize a protein of interest at a subcellular level in C. elegans lysates, which can give insight into the location, function and/or toxicity of proteins.

U2 - 10.21769/BioProtoc.3053

DO - 10.21769/BioProtoc.3053

M3 - Article

C2 - 30467549

SN - 1929-0748

VL - 8

JO - JMIR research protocols

JF - JMIR research protocols

IS - 20

M1 - 3053

ER -

Nuclear/Cytoplasmic Fractionation of Proteins from Caenorhabditis elegans

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