Optimised microcomputer-guided quantitative microradiography on dental mineralised tissue slices

E. de Josselin de Jong*, J. J. ten Bosch, J. Noordmans

*Corresponding author for this work

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    It is well realised that the contact microradiographic method is the most direct method that gives position-dependent information on the mineral content of calcified tissue and its loss. The authors developed a microcomputer-guided microradiographic system which features fast operation by the experimenter with a low appearance of errors. Tooth tissue slices of 75 mu m thickness are cut with a sawing machine. Images (Cu K alpha radiation) of the tooth slice and an aluminium step wedge (exposure 10-15 s) are made on film (Kodak SO-253). The images of step wedge and tooth slice are scanned in a densitometer (1 mu m*30 mu m slit), which is fitted with an XY table (0.5 mu m steps). A microcomputer (Apple IIe) is programmed to control the XY table and to record the optical film transmission. Scans of the images are plotted on the computer screen. On the basis of the analysis of random errors and a comparison with chemical analysis of tooth slices the authors claim that the error in mineral volume percentage amounts to 4% of its value. Starting with a microradiographic image of a tooth slice 5 min are required to obtain a microradiographic curve on paper and to obtain a value for mineral loss
    Original languageEnglish
    Pages (from-to)887-899
    Number of pages13
    JournalPhysics in Medicine and Biology
    Issue number7
    Publication statusPublished - Jul-1987

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