Organic field-effect transistor-based biosensors functionalized with protein receptors

Francesco Maddalena; Marjon J. Kuiper; Bert Poolman; Frank Brouwer; Jan C. Hummelen; Dago M. de Leeuw; Bert  de Boer; Paul W. M. Blom

doi:10.1063/1.3518681

Organic field-effect transistor-based biosensors functionalized with protein receptors

Francesco Maddalena^*, Marjon J. Kuiper, Bert Poolman, Frank Brouwer, Jan C. Hummelen, Dago M. de Leeuw, Bert de Boer, Paul W. M. Blom

^*Corresponding author for this work

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

An organic field-effect transistor with integrated proteins (Bio-FET) for sensing of sulfate ions is presented. A sulfate receptor was engineered to contain a thiol group for surface-anchoring without affecting its binding activity. The modified receptor was covalently coupled to a maleimide-functionalized polystyrene layer, and integrated as gate dielectric in a dual-gate transducer. The binding of sulfate ions in dry conditions was detected by a shift in the threshold voltage. Combined with surface density measurements by atomic force microscopy , an effective charge of −1.7q per protein was found, as expected from the Bio-FET operation model.

Original language	English
Article number	124501
Pages (from-to)	124501-1-124501-4
Number of pages	4
Journal	Journal of Applied Physics
Volume	108
Issue number	12
DOIs	https://doi.org/10.1063/1.3518681
Publication status	Published - 15-Dec-2010

Keywords

THIN-FILM TRANSISTORS
TRANSPORT
SULFATE

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title = "Organic field-effect transistor-based biosensors functionalized with protein receptors",

abstract = "An organic field-effect transistor with integrated proteins (Bio-FET) for sensing of sulfate ions is presented. A sulfate receptor was engineered to contain a thiol group for surface-anchoring without affecting its binding activity. The modified receptor was covalently coupled to a maleimide-functionalized polystyrene layer, and integrated as gate dielectric in a dual-gate transducer. The binding of sulfate ions in dry conditions was detected by a shift in the threshold voltage. Combined with surface density measurements by atomic force microscopy , an effective charge of −1.7q per protein was found, as expected from the Bio-FET operation model.",

keywords = "THIN-FILM TRANSISTORS, TRANSPORT, SULFATE",

author = "Francesco Maddalena and Kuiper, {Marjon J.} and Bert Poolman and Frank Brouwer and Hummelen, {Jan C.} and {de Leeuw}, {Dago M.} and {de Boer}, Bert and Blom, {Paul W. M.}",

note = "Relation: http://www.rug.nl/research/stratingh/ date_submitted:2010 Rights: University of Groningen, Stratingh Institute for Chemistry",

year = "2010",

month = dec,

day = "15",

doi = "10.1063/1.3518681",

language = "English",

volume = "108",

pages = "124501--1--124501--4",

journal = "Journal of Applied Physics",

issn = "0021-8979",

publisher = "AMER INST PHYSICS",

number = "12",

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TY - JOUR

T1 - Organic field-effect transistor-based biosensors functionalized with protein receptors

AU - Maddalena, Francesco

AU - Kuiper, Marjon J.

AU - Poolman, Bert

AU - Brouwer, Frank

AU - Hummelen, Jan C.

AU - de Leeuw, Dago M.

AU - de Boer, Bert

AU - Blom, Paul W. M.

N1 - Relation: http://www.rug.nl/research/stratingh/ date_submitted:2010 Rights: University of Groningen, Stratingh Institute for Chemistry

PY - 2010/12/15

Y1 - 2010/12/15

N2 - An organic field-effect transistor with integrated proteins (Bio-FET) for sensing of sulfate ions is presented. A sulfate receptor was engineered to contain a thiol group for surface-anchoring without affecting its binding activity. The modified receptor was covalently coupled to a maleimide-functionalized polystyrene layer, and integrated as gate dielectric in a dual-gate transducer. The binding of sulfate ions in dry conditions was detected by a shift in the threshold voltage. Combined with surface density measurements by atomic force microscopy , an effective charge of −1.7q per protein was found, as expected from the Bio-FET operation model.

AB - An organic field-effect transistor with integrated proteins (Bio-FET) for sensing of sulfate ions is presented. A sulfate receptor was engineered to contain a thiol group for surface-anchoring without affecting its binding activity. The modified receptor was covalently coupled to a maleimide-functionalized polystyrene layer, and integrated as gate dielectric in a dual-gate transducer. The binding of sulfate ions in dry conditions was detected by a shift in the threshold voltage. Combined with surface density measurements by atomic force microscopy , an effective charge of −1.7q per protein was found, as expected from the Bio-FET operation model.

KW - THIN-FILM TRANSISTORS

KW - TRANSPORT

KW - SULFATE

U2 - 10.1063/1.3518681

DO - 10.1063/1.3518681

M3 - Article

VL - 108

SP - 124501-1-124501-4

JO - Journal of Applied Physics

JF - Journal of Applied Physics

SN - 0021-8979

IS - 12

M1 - 124501

ER -