Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR

AR Neves; WA Pool; J Kok; OP Kuipers; H Santos; Ana Rute Neves; Wietske A. Pool

doi:10.1016/j.fmrre.2005.04.005

Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR

AR Neves
, WA Pool
, J Kok
, OP Kuipers
, H Santos^*
, Ana Rute Neves
, Wietske A. Pool

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

192 Citations (Scopus)

91 Downloads (Pure)

Abstract

The wide application of lactic acid bacteria in the production of fermented foods depends to a great extent on the unique features of sugar metabolism in these organisms. The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks. In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse. Examples of the application of this methodology to identify metabolic bottlenecks and regulatory sites are presented. The use of this information to direct metabolic engineering strategies is illustrated. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

Original language	English
Pages (from-to)	531-554
Number of pages	24
Journal	FEMS Microbiology Reviews
Volume	29
Issue number	3
DOIs	https://doi.org/10.1016/j.fmrre.2005.04.005
Publication status	Published - Aug-2005

Keywords

LAB
Lactococcus lactis
in vivo NMR
glycolysis regulation
metabolic engineering
NUCLEAR-MAGNETIC-RESONANCE
PYRUVATE-FORMATE-LYASE
L-LACTATE DEHYDROGENASE
STREPTOCOCCUS-LACTIS
ACID BACTERIA
GLUCOSE-METABOLISM
SACCHAROMYCES-CEREVISIAE
PRODUCT FORMATION
SUBSP LACTIS
PHOSPHOTRANSFERASE SYSTEM

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@article{16b0fa406e9846c0b08182d3f5324086,

title = "Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR",

abstract = "The wide application of lactic acid bacteria in the production of fermented foods depends to a great extent on the unique features of sugar metabolism in these organisms. The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks. In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse. Examples of the application of this methodology to identify metabolic bottlenecks and regulatory sites are presented. The use of this information to direct metabolic engineering strategies is illustrated. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.",

keywords = "LAB, Lactococcus lactis, in vivo NMR, glycolysis regulation, metabolic engineering, NUCLEAR-MAGNETIC-RESONANCE, PYRUVATE-FORMATE-LYASE, L-LACTATE DEHYDROGENASE, STREPTOCOCCUS-LACTIS, ACID BACTERIA, GLUCOSE-METABOLISM, SACCHAROMYCES-CEREVISIAE, PRODUCT FORMATION, SUBSP LACTIS, PHOSPHOTRANSFERASE SYSTEM",

author = "AR Neves and WA Pool and J Kok and OP Kuipers and H Santos and Neves, \{Ana Rute\} and Pool, \{Wietske A.\}",

note = "Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780-156 Oeiras, PortugalFAU - Neves, Ana Rute",

year = "2005",

month = aug,

doi = "10.1016/j.fmrre.2005.04.005",

language = "English",

volume = "29",

pages = "531--554",

journal = "FEMS Microbiology Reviews",

issn = "0168-6445",

publisher = "Oxford University Press",

number = "3",

}

TY - JOUR

T1 - Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR

AU - Neves, AR

AU - Pool, WA

AU - Kok, J

AU - Kuipers, OP

AU - Santos, H

AU - Neves, Ana Rute

AU - Pool, Wietske A.

N1 - Instituto de Tecnologia Quimica e Biologica, Universidade Nova de Lisboa, Rua da Quinta Grande, 6, Apt 127, 2780-156 Oeiras, PortugalFAU - Neves, Ana Rute

PY - 2005/8

Y1 - 2005/8

N2 - The wide application of lactic acid bacteria in the production of fermented foods depends to a great extent on the unique features of sugar metabolism in these organisms. The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks. In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse. Examples of the application of this methodology to identify metabolic bottlenecks and regulatory sites are presented. The use of this information to direct metabolic engineering strategies is illustrated. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

AB - The wide application of lactic acid bacteria in the production of fermented foods depends to a great extent on the unique features of sugar metabolism in these organisms. The relative metabolic simplicity and the availability of genetic tools made Lactococcus lactis the organism of choice to gain insight into metabolic and regulatory networks. In vivo nuclear magnetic resonance has proven a very useful technique to monitor non-invasively the dynamics of intracellular metabolite and co-factor pools following a glucose pulse. Examples of the application of this methodology to identify metabolic bottlenecks and regulatory sites are presented. The use of this information to direct metabolic engineering strategies is illustrated. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

KW - LAB

KW - Lactococcus lactis

KW - in vivo NMR

KW - glycolysis regulation

KW - metabolic engineering

KW - NUCLEAR-MAGNETIC-RESONANCE

KW - PYRUVATE-FORMATE-LYASE

KW - L-LACTATE DEHYDROGENASE

KW - STREPTOCOCCUS-LACTIS

KW - ACID BACTERIA

KW - GLUCOSE-METABOLISM

KW - SACCHAROMYCES-CEREVISIAE

KW - PRODUCT FORMATION

KW - SUBSP LACTIS

KW - PHOSPHOTRANSFERASE SYSTEM

U2 - 10.1016/j.fmrre.2005.04.005

DO - 10.1016/j.fmrre.2005.04.005

M3 - Review article

SN - 0168-6445

VL - 29

SP - 531

EP - 554

JO - FEMS Microbiology Reviews

JF - FEMS Microbiology Reviews

IS - 3

ER -

Overview on sugar metabolism and its control in Lactococcus lactis - The input from in vivo NMR

Abstract

Keywords

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