p53 Prohibits Propagation of Chromosome Segregation Errors that Produce Structural Aneuploidies

Mar Soto, Jonne A Raaijmakers, Bjorn Bakker, Diana Spierings, Peter Lansdorp, Floris Foijer, René H Medema

Research output: Contribution to journalArticleAcademicpeer-review

55 Citations (Scopus)
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Abstract

The presence of an abnormal karyotype has been shown to be profoundly detrimental at the cellular and organismal levels but is an overt hallmark of cancer. Aneuploidy can lead to p53 activation and thereby prevents proliferation, but the exact trigger for p53 activation has remained controversial. Here, we have used a system to induce aneuploidy in untransformed human cells to explore how cells deal with different segregation errors. We show that p53 is activated only in a subset of the cells with altered chromosome content. Importantly, we find that at least a subset of whole-chromosome aneuploidies can be propagated in p53-proficient cells, indicating that aneuploidy does not always lead to activation of p53. Finally, we demonstrate that propagation of structural aneuploidies (gain or loss of part of a chromosome) induced by segregation errors is limited to p53-deficient cells.

Original languageEnglish
Pages (from-to)2423-2431
Number of pages9
JournalCell reports
Volume19
Issue number12
DOIs
Publication statusPublished - 20-Jun-2017

Keywords

  • DNA-DAMAGE
  • STEM-CELLS
  • CANCER
  • PROLIFERATION
  • TUMORIGENESIS
  • INSTABILITY
  • INHIBITOR
  • GSK923295
  • MURINE

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